NDLI: Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology

Content Provider	IEEE Xplore Digital Library
Author	Breen, P.P. Buskila, Y.
Copyright Year	2014
Description	Author affiliation: MARCS Inst., Univ. of Western Sydney, Penrith, NSW, Australia (Breen, P.P.; Buskila, Y.)
Abstract	In vitro brain slice preparations are instrumental in developing our understanding of the nervous system. However, the current lifespan of an acute brain slice is limited to approximately 6-12 hours. This reduces potential experimentation time and leads to considerable waste of neural tissue. We have designed, developed and tested a novel incubation system capable of extending the lifespan of these brain slices. This is done by controlling the temperature and pH of the artificial cerebral spinal fluid in which the slices are incubated while continuously passing the fluid through a UVC filtration system. This system is capable of maintaining extremely low bacterial levels and significantly extending the brain slice lifespan to at least 24 hours. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays.
Sponsorship	IEEE Eng. Med. Biol. Soc.
Starting Page	4864
Ending Page	4867
File Size	870269
Page Count	4
File Format	PDF
ISBN	9781424479290
ISSN	1557170X
DOI	10.1109/EMBC.2014.6944713
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-08-26
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Brain Microorganisms Neurons In vitro Mice Immune system
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Neuroengineering: Building interfaces from neurons to brain

An in vitro system for modeling brain reactive responses and changes in neuroprosthetic device impedance

Modeling the Blood-Brain Barrier in a 3D triple co-culture microfluidic system

Computer-based automatic identification of neurons in gigavoxel-sized 3D human brain images

Reducing human error in P300 speller paradigm for brain-computer interface

A Brain-Computer Interface (BCI) system to use arbitrary Windows applications by directly controlling mouse and keyboard

Morphological descriptors for automatic detection of epileptiform events

Neurophysiology study of early visual processing of face and non-face recognition under simulated prosthetic vision

Modeling of deep breath vasoconstriction reflex

Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology

Similar Documents

Neuroengineering: Building interfaces from neurons to brain

An in vitro system for modeling brain reactive responses and changes in neuroprosthetic device impedance

Modeling the Blood-Brain Barrier in a 3D triple co-culture microfluidic system

Computer-based automatic identification of neurons in gigavoxel-sized 3D human brain images

Reducing human error in P300 speller paradigm for brain-computer interface

A Brain-Computer Interface (BCI) system to use arbitrary Windows applications by directly controlling mouse and keyboard

Morphological descriptors for automatic detection of epileptiform events

Neurophysiology study of early visual processing of face and non-face recognition under simulated prosthetic vision

Modeling of deep breath vasoconstriction reflex

Braincubator: An incubation system to extend brain slice lifespan for use in neurophysiology