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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kim, Jin-Young Kim, Hyun Jung Kim, Sung-Moo Park, Kyung-Ran Jang, Hyeung-Jin Lee, Eun Ha Jung, Sang Hoon Ahn, Kwang Seok |
| Description | Author Affiliation: Kim JY ( Department of Oriental Pathology, College of Oriental Medicine and Institute of Oriental Medicine, Kyung Hee University, 1 Hoegidong Dongdaemungu, Seoul 130-701, Republic of Korea.) |
| Abstract | AIM OF THE STUDY: Thuja orientalis (TO) has been a recognized herbal medicine across Northeast Asian countries for thousands of years and used for the treatment of various inflammatory diseases through as yet undefined mechanisms. In this study, we set out to determine whether the anti-inflammatory effects of this plant are mediated to suppress mitogen-activated protein kinases (MAPKs) and nuclear factor-κB (NF-κB) activation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. MATERIALS AND METHODS: RAW 264.7 cells were pretreated with the methylene chloride fraction of TO (MTO) and stimulated with LPS. Nitric oxide (NO) release was determined by the accumulation of nitrite in the culture supernatants and tumor necrosis factor- (TNF- ) and IL-6 secretion were determined by immunoenzymatic assay. Inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression were evaluated via RT-PCR and Western blotting. NF-κB activation was also evaluated by reporter gene assay and electrophoretic mobility shift assay (EMSA). In addition, the protective effect of MTO was evaluated by use of the LPS-induced endotoxin shock model in mice. RESULTS: We found that MTO significantly suppressed LPS-stimulated NO and IL-6 production without affecting cell viability. MTO inhibited the expression of LPS-induced iNOS and COX-2 protein and their mRNA expression. Also, TNF- and IL-6 secretion were decreased by MTO in both PMA and ionomycin-stimulated splenocytes. As a result, MTO inhibited pro-inflammatory cytokines such as TNF- and IL-6, which is hypothesized as being due to the suppression of LPS-induced p38 MAPK and NF-κB activation. Moreover, MTO improved the survival rate during lethal endotoxemia by inhibiting the production of TNF- in an animal model and our LC-MS analysis showed that a major component of MTO was pinusolide. CONCLUSIONS: We demonstrate here the evidence that the methylene chloride fraction of Thuja orientalis (MTO) potentially inhibits the biomarkers related to inflammation in vitro and in vivo, and might be provided as a potential candidate for the treatment of inflammatory diseases. |
| File Format | HTM / HTML |
| ISSN | 03788741 |
| Issue Number | 2 |
| Volume Number | 133 |
| e-ISSN | 18727573 |
| Journal | Journal of Ethnopharmacology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-01-27 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Ethnopharmacology Anti-inflammatory Agents Pharmacology Endotoxemia Prevention & Control Nf-kappa B Antagonists & Inhibitors Thuja P38 Mitogen-activated Protein Kinases Animals Isolation & Purification Base Sequence Biological Markers Metabolism Cell Line Cyclooxygenase 2 Genetics Dna Primers Ethnopharmacology Herbal Medicine Interleukin-6 Biosynthesis Lipopolysaccharides Toxicity Male Methylene Chloride Mice Mice, Inbred Balb C Nitric Oxide Nitric Oxide Synthase Type Ii Plant Leaves Chemistry Plant Preparations Rna, Messenger Signal Transduction Drug Effects Tumor Necrosis Factor-alpha Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmacology |
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