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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kim, Tae-Hee Li, Hua Wu, Qian Lee, Hwa Jin Ryu, Jae-Ha |
| Description | Author Affiliation: Kim TH ( Research Center for Cell Fate Control and College of Pharmacy, Sookmyung Women's University, Yongsan-Gu, Seoul 140-742, Republic of Korea.) |
| Abstract | UNLABELLED: ETHONOPHARMACOLOGICAL RELEVANCE: Thuja orientalis (L) ENDL (Cupressaceae) is an evergreen arbor that is distributed throughout Northeast Asia. This plant has been used as a traditional medicine for the treatment of various inflammatory diseases such as dermatitis, gout and chronic tracheitis. AIM OF THE STUDY: To isolate the active principles responsible for the anti-inflammatory activities of Thuja orientalis and to disclose their mechanism of action in lipopolysaccharide (LPS)-stimulated macrophages. MATERIALS AND METHODS: The methanolic leaves and stem extracts of the Thuja orientalis were successively fractionated into EtOAc, n-BuOH and the remaining aqueous fractions. The EtOAc soluble fraction, which exhibited significant inhibitory activity on LPS-induced nitric oxide (NO) production, was followed by successive activity-guided chromatography to yield seven diterpenoids. The anti-inflammatory properties of active constituents were evaluated by RT-PCR, Western blotting and reporter gene assay in cell culture system. RESULTS: A new labdane diterpene, 15-nor-14-oxolabda-8(17),13(16)-dien-19-oic acid (1), and six known diterpenoids (2-7) were isolated from the EtOAc extracts of Thuja orientalis. The isolated compounds 1-7 were evaluated for the inhibitory activity of LPS-induced NO production in RAW264.7 macrophage cells. Compound 1 was the most potent among the isolated compounds for the inhibition of LPS-induced NO production (IC501: 3.56µM). Compound 1 reduced the production of pro-inflammatory mediators and suppressed the expression of the inflammatory enzymes of iNOS and COX-2. In addition, compound 1 attenuated the LPS-induced transcriptional activity of NF-κB by the inhibition of inhibitory-κB degradation, and suppressed the activity of extracellular signal regulated kinase (ERK). CONCLUSIONS: This study demonstrates that a new labdane diterpene from Thuja orientalis inhibits the inflammatory responses by the suppression of NF-κB activity and ERK phosphorylation. This compound might be a valuable candidate for the development of anti-inflammatory drugs. |
| File Format | HTM / HTML |
| ISSN | 03788741 |
| Issue Number | 3 |
| Volume Number | 146 |
| e-ISSN | 18727573 |
| Journal | Journal of Ethnopharmacology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-04-19 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Ethnopharmacology Anti-inflammatory Agents Pharmacology Diterpenes Plant Extracts Thuja Chemistry Animals Isolation & Purification Blotting, Western Cell Culture Techniques Cell Line Cytokines Antagonists & Inhibitors Biosynthesis Immunology Dinoprostone Lipopolysaccharides Macrophages Drug Effects Enzymology Mice Molecular Structure Nitric Oxide Plant Leaves Plant Stems Reverse Transcriptase Polymerase Chain Reaction Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmacology |
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