NDLI: Stimulation of osteoblastic differentiation and mineralization in MC3T3-E1 cells by antler and fermented antler using Cordyceps militaris.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, Hyun-Sun Kim, Min Kyung Kim, Yoo-Kyung Jung, Eun Young Park, Chul Soo Woo, Moon Jea Lee, Sang Hun Kim, Jin Soo Suh, Hyung Joo
Spatial Coverage	Republic of Korea
Description	Author Affiliation: Lee HS ( Department of Food and Nutrition, Korea University, Seoul 136-703, Republic of Korea.)
Abstract	ETHNOPHARMACOLOGICAL RELEVANCE: For thousands of years antlers have been used in Asian countries to promote rapid healing, treat weight loss, slow growth in children, strengthen weak bones, and alleviate cold hands and feet. AIM OF THE STUDY: The present study was performed to examine the effect of fermentation on the ability of antler to act as a stimulator of bone growth. MATERIALS AND METHODS: This study used pre-osteoblast MC3T3-E1 cells to examine factors related to bone growth, such as cell proliferation, production of alkaline phosphatase (ALP), and deposition of extracellular matrix proteins (e.g., collagens, osteonectin, bone sialoprotein (BSP)), via the treatment of non-fermented and fermented antler. RESULTS: Antler fermentation using Cordyceps militaris was carried out at 25°C for seven days. The total content of sugar, sialic acid, and protein increased with fermentation time. Cell proliferation was greater in the fermented antler- (FA-) treated groups than in the NFA- (non-fermented antler-) treated groups, in which proliferation increased significantly up to 137% of the basal value. Significant increases in mRNA expression and ALP activity were found at FA concentrations of 50-100 µg/ml; at 100 µg/ml the activity had increased 119% compared to the control activity. For NFA and FA the expression levels of type I collagen mRNA significantly increased in a dose-dependent manner at all treatment doses. However, significant differences between the antler groups were not observed. Mineralization significantly increased by NFA and FA treatment to 183% and 241%, respectively, when compared to colostrum, as a positive control (165%). CONCLUSIONS: Antler treatment increased the proliferation of osteoblasts and bone matrix proteins, such as type I collagen and BSP. Antler fermented with Cordyceps militaris showed enhanced activity, and its stimulatory effects on cell proliferation and ALP production were greater than those of NFA. We surmise that these increases in activity were related to increased sialic acid content. Therefore, the results of this study suggest that the physiological effects of antler, including bone growth, may be increased through the fermentation process.
File Format	HTM / HTML
ISSN	03788741
Issue Number	2
Volume Number	133
e-ISSN	18727573
Journal	Journal of Ethnopharmacology
Language	English
Publisher	Elsevier
Publisher Date	2011-01-27
Publisher Place	Ireland
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Ethnopharmacology Antlers Chemistry Cordyceps Metabolism Osteoblasts Cytology 3t3 Cells Alkaline Phosphatase Genetics Animals Base Sequence Bone Development Calcium Cell Differentiation Cell Proliferation Deer Ethnopharmacology Extracellular Matrix Proteins Fermentation Medicine, Korean Traditional Mice Rna, Messenger Republic Of Korea Journal Article
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Pharmacology

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