NDLI: The WNT/ß-catenin pathway mediates the anti-adipogenic mechanism of SH21B, a traditional herbal medicine for the treatment of obesity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, Haeyong Bae, Sungmin Yoon, Yoosik
Spatial Coverage	Republic of Korea
Description	Author Affiliation: Lee H ( Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.)
Abstract	AIM OF THE STUDY: This study was conducted to elucidate the molecular mechanisms of SH21B, a traditional Korean herbal medicine commonly used for the treatment of obesity. MATERIALS AND METHODS: 3T3-L1 preadipocytes were differentiated into adipocytes in the presence or absence of SH21B. Changes in mRNA or protein levels were analyzed using microarray, real-time polymerase chain reaction and western blotting analyses. Small interference (si)RNA transfection experiments were conducted to elucidate the essential role of ß-catenin. RESULTS: Microarray analyses showed that components of the WNT/ß-catenin pathway including ß-catenin, cyclin D1 and dishevelled 2 were up-regulated more than two-fold as a result of SH21B treatment during adipogenesis, which were confirmed by real-time PCR and western blotting. Modulation of the WNT/ß-catenin pathway by SH21B resulted in the nuclear accumulation of ß-catenin. Both intracellular lipid droplet formation and expressions of adipogenic genes including PPARÎ³, C/EBP , FABP4 and LPL, which were inhibited by SH21B, were significantly recovered by ß-catenin siRNA transfection. CONCLUSIONS: SH21B modulates components of the WNT/ß-catenin pathway during adipogenesis, and ß-catenin plays a crucial role in the anti-adipogenic mechanism of SH21B.
File Format	HTM / HTML
ISSN	03788741
Issue Number	2
Volume Number	133
e-ISSN	18727573
Journal	Journal of Ethnopharmacology
Language	English
Publisher	Elsevier
Publisher Date	2011-01-27
Publisher Place	Ireland
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Ethnopharmacology Obesity Drug Therapy Metabolism Phytotherapy Plant Preparations Pharmacology Wnt Proteins Beta Catenin 3t3-l1 Cells Adipocytes Cytology Drug Effects Adipogenesis Animals Cell Differentiation Ethnopharmacology Humans Medicine, Korean Traditional Mice Oligonucleotide Array Sequence Analysis Rna, Messenger Genetics Rna, Small Interfering Republic Of Korea Signal Transduction Antagonists & Inhibitors Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Pharmacology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

SH21B, an anti-obesity herbal composition, inhibits fat accumulation in 3T3-L1 adipocytes and high fat diet-induced obese mice through the modulation of the adipogenesis pathway.

Nucleoredoxin promotes adipogenic differentiation through regulation of Wnt/ß-catenin signaling.

Cornus kousa F.Buerger ex Miquel increases glucose uptake through activation of peroxisome proliferator-activated receptor Î³ and insulin sensitization.

Herbal composition Gambigyeongsinhwan (4) from Curcuma longa, Alnus japonica, and Massa Medicata Fermentata inhibits lipid accumulation in 3T3-L1 cells and regulates obesity in Otsuka Long-Evans Tokushima Fatty rats.

The anti-angiogenic herbal extract from Melissa officinalis inhibits adipogenesis in 3T3-L1 adipocytes and suppresses adipocyte hypertrophy in high fat diet-induced obese C57BL/6J mice.

Anti-adipogenic activity of blue mussel (Mytilus edulis) extract by regulation of 3T3-L1 adipogenesis through Wnt/β-catenin signaling pathway

Traditional Herbal Formula Oyaksungi-San Inhibits Adipogenesis in 3T3-L1 Adipocytes

Trans10, cis12 conjugated linoleic acid inhibits 3T3-L1 adipocyte adipogenesis by elevating β-catenin levels

Analysis of traditional knowledge about medicinal plants utilized in communities of Jirisan National Park (Korea).

The WNT/ß-catenin pathway mediates the anti-adipogenic mechanism of SH21B, a traditional herbal medicine for the treatment of obesity.

Similar Documents

SH21B, an anti-obesity herbal composition, inhibits fat accumulation in 3T3-L1 adipocytes and high fat diet-induced obese mice through the modulation of the adipogenesis pathway.

Nucleoredoxin promotes adipogenic differentiation through regulation of Wnt/ß-catenin signaling.

Cornus kousa F.Buerger ex Miquel increases glucose uptake through activation of peroxisome proliferator-activated receptor Î³ and insulin sensitization.

Herbal composition Gambigyeongsinhwan (4) from Curcuma longa, Alnus japonica, and Massa Medicata Fermentata inhibits lipid accumulation in 3T3-L1 cells and regulates obesity in Otsuka Long-Evans Tokushima Fatty rats.

The anti-angiogenic herbal extract from Melissa officinalis inhibits adipogenesis in 3T3-L1 adipocytes and suppresses adipocyte hypertrophy in high fat diet-induced obese C57BL/6J mice.

Anti-adipogenic activity of blue mussel (Mytilus edulis) extract by regulation of 3T3-L1 adipogenesis through Wnt/β-catenin signaling pathway

Traditional Herbal Formula Oyaksungi-San Inhibits Adipogenesis in 3T3-L1 Adipocytes

Trans10, cis12 conjugated linoleic acid inhibits 3T3-L1 adipocyte adipogenesis by elevating β-catenin levels

Analysis of traditional knowledge about medicinal plants utilized in communities of Jirisan National Park (Korea).

The WNT/ß-catenin pathway mediates the anti-adipogenic mechanism of SH21B, a traditional herbal medicine for the treatment of obesity.