NDLI: Cornus kousa F.Buerger ex Miquel increases glucose uptake through activation of peroxisome proliferator-activated receptor Î³ and insulin sensitization.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Daeyoung Park, Kwang-Kyun Lee, Sang Kook Lee, Seung-Eun Hwang, Jae-Kwan
Spatial Coverage	Republic of Korea
Description	Author Affiliation: Kim D ( Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea.)
Abstract	AIM OF THE STUDY: Cornus kousa F.Buerger ex Miquel, an oriental medicinal plant, has been traditionally used for the treatment of hyperglycemia, but its molecular mechanism remains unknown. The goal of this study was to investigate the peroxisome proliferator-activated receptor Î³ (PPARÎ³) ligand-binding activity of Cornus kousa and to determine the effects of Cornus kousa on insulin sensitization in 3T3-L1 cells for the treatment of type 2 diabetes. MATERIALS AND METHODS: PPARÎ³ luciferase transactivation assay was used to evaluate the PPARÎ³ ligand-binding activity of Cornus kousa leaf extract. Western blot analysis, oil Red O staining, and glucose uptake assay were performed to evaluate PPARÎ³ agonistic activity and insulin sensitizing effects of Cornus kousa leaf extract (CKE) in 3T3-L1 cells. RESULTS: CKE increased PPARÎ³ ligand-binding activity in a dose-dependent manner. In addition, CKE enhanced adipogenesis and the expression of PPARÎ³ target proteins, including glucose transporter 4 (GLUT4) and adiponectin, as well as proteins involved in adipogenesis, including PPARÎ³ and CCAAT/enhancer binding protein (C/EBP ) in 3T3-L1 adipocytes. Furthermore, CKE led to significant induction of glucose uptake and stimulated insulin signaling, but not to activation of AMP-activated protein kinase (AMPK) signaling. The enhanced glucose uptake by CKE were abolished by treatment with bisphenol a diglycidyl ether (BADGE), a PPARÎ³ antagonist, or LY294002, an inhibitor of phosphoinositide 3-kinase (PI3K), but not by compound C, an AMPK inhibitor. CONCLUSION: Consistent with the high PPARÎ³ ligand-binding activity, CKE increased glucose uptake through PPARÎ³ activation and insulin signaling. These results suggest that CKE could have pharmacological effects for the treatment of hyperglycemia and type 2 diabetes.
File Format	HTM / HTML
ISSN	03788741
Issue Number	2
Volume Number	133
e-ISSN	18727573
Journal	Journal of Ethnopharmacology
Language	English
Publisher	Elsevier
Publisher Date	2011-01-27
Publisher Place	Ireland
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Ethnopharmacology Cornus Insulin Metabolism Ppar Gamma Plant Preparations Pharmacology 3t3-l1 Cells Adipogenesis Drug Effects Animals Cos Cells Cercopithecus Aethiops Diabetes Mellitus, Type 2 Drug Therapy Ethnopharmacology Glucose Humans Hyperglycemia Ligands Medicine, Korean Traditional Mice Phytotherapy Administration & Dosage Plants, Medicinal Republic Of Korea Signal Transduction Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Pharmacology

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