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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Zhongcheng Gong Nagarajan, K. Penmetsa, S. Mills, D. Long Que |
| Copyright Year | 2009 |
| Description | Author affiliation: Institute for Micromanufacturing, Louisiana Tech University, USA (Zhongcheng Gong; Penmetsa, S.; Mills, D.; Long Que) || School of Biological Science, Louisiana Tech University, USA (Nagarajan, K.) |
| Abstract | A patch-clamp (PC) microchip with integrated air-bubble actuators (AB actuators) for cell positioning is demonstrated, showing the promise for an automated PC microsystem. AB actuators are pneumatic actuators. The air pressure is generated by PDMS membrane mechanical deformation. By integrating with microfluidic channels and PC electrodes, AB actuators can potentially help avoid drawbacks of random cell selection or positioning for a PC microchip, paving the way for an automated PC microsystem. In this effort, the lateral electrode-pipette, located opposite to the outlet of an AB actuator, is designed for trapping a cell. The positioning and capturing of 3T3 cells were demonstrated successfully. Measurements found that the electrode impedance is 1MΩ the seal resistance is 5MΩ with a captured cell. Optimization of the size and shape of the pipette is underway to enhance the seal resistance. |
| Starting Page | 278 |
| Ending Page | 281 |
| File Size | 6482227 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781424446292 |
| DOI | 10.1109/NEMS.2009.5068577 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2009-01-05 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Actuators patch-clamp electrodes air bubble actuator microfluidics patch-clamp device |
| Content Type | Text |
| Resource Type | Article |
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