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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Huiqi He Chang, D.C. Yi-Kuen Lee |
| Copyright Year | 2009 |
| Description | Author affiliation: Bioengineering Graduate Program, The Hong Kong University of Science and Technology, Hong Kong (Huiqi He) || Dept of Mechanical Engineering, The Hong Kong University of Science and Technology, Hong Kong (Yi-Kuen Lee) || Dept of Biology, The Hong Kong University of Science and Technology, Hong Kong (Chang, D.C.) A battery-powered miniaturized electroporation (EP) system was designed and fabricated to tackle the challenging DNA transfection of stem cells. The system consists of a programmable mini-electroporator and a micro EP chip. Large statistical data of loading efficiency for five different size molecules and cell viability were determined under various voltage and duration in this system for HeLa cell and MEF cell line. Based on more than 2,000 single-cell electroporation experiments, an EP phase diagram for MEF cells have been generated for the first time. It can be used to significantly increase the in-situ gene transfer efficiency. In addition, the EP boundary curve for MEF cells can be described by a simple empirical formula. Comparing to the conventional instrument, the miniaturized electroporation system can greatly shorten the experimental time. We also proposed a new system for continuous online monitoring in-situ gene expression, which is still in progress, |
| Starting Page | 147 |
| Ending Page | 151 |
| File Size | 7600928 |
| Page Count | 5 |
| File Format | |
| ISBN | 9781424446292 |
| DOI | 10.1109/NEMS.2009.5068546 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2009-01-05 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Stem cells Cells (biology) DNA Embryo Gold Pulsed power supplies Instruments Monitoring Safety Fabrication stem cell Electroporation phase diagram MEF cells in-situ gene transfer |
| Content Type | Text |
| Resource Type | Article |
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