NDLI: Disposable Smart Immunosensing Biochip Using a Novel Electrochemical signaling Method

Content Provider	IEEE Xplore Digital Library
Author	Seok Hui Im Sin Wook Park Jun Hwang Lee Hyoung Kil Choi Yoon, H.C. Sang Sik Yang
Copyright Year	2006
Description	Author affiliation: Sch. of Electron. & Comput. Eng., Ajou Univ., Suwon (Seok Hui Im; Sin Wook Park)
Abstract	This paper presents a smart immunosensing biochip using a novel electrochemical method for the portable clinical diagnostics system. The fluid flow of this device can be induced by capillary attraction force only and controlled by a geometrical structure. By this fluid transport mechanism, it does not require external electrical power or control circuit parts. Therefore it is suitable for disposable and portable lab-on-a-chip applications. The fabricated biochip consists of the two poly(dimethyl siloxane) (PDMS) layers and a glass substrate for immunoelectrodes. The size of the biochip is 25times20times6 $mm^{3}.$ The signal amplification strategy applied in this biochip is based on the back-filling immobilization of biocatalytic enzyme at immunoelectrodes, circumventing the use of enzyme-labeled antibody. For the immunosensing surface construction, a poly(amidoamine) G4-dendrimer was employed as a matrix for ligand functionalization. As a model biorecognition reaction, the dinitrophenyl (DNP) antigen-functionalized electrode was fabricated and an anti-DNP antibody was used. Glucose oxidase (GOX) was chosen to amplify electrochemical signal by enzymatic catalysis. The non-labeled native antibody was biospecfically bound to the immobilized ligand, and the activated enzyme (periodate-treated GOX) reacted and back-filled the remaining surface amine group on the dendrimer layer
Starting Page	1169
Ending Page	1173
File Size	5177579
Page Count	5
File Format	PDF
ISBN	1424401399
DOI	10.1109/NEMS.2006.334673
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-01-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Immune system Force control Fluid flow control Biochemistry Fluid flow Circuits Lab-on-a-chip Glass Electrodes Sugar glucose oxidase immunosensing capillary attraction force PDMS back-filling
Content Type	Text
Resource Type	Article

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