NDLI: Simultaneous Quantification for Hepatitis B Virus and Hepatitis C Virus Using Real-time PCR Lab-on-a-chip

Content Provider	IEEE Xplore Digital Library
Author	Chien, J.H. Lee, D.S. Chou, W.P. Wang, P.Y. Yang, C.R. Wu, M.H. Tsai, C.Y. Chang, T.L. Lee, Y.W. Cheng, Y.T. Chen, P.H.
Copyright Year	2006
Description	Author affiliation: Dept. of Eng., National Taiwan Univ., Taipei (Chien, J.H.)
Abstract	The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-chip system, for the dispersion of the fluorescence in the micro fluidic channels, the received fluorescence is much lower than the emitted. To enhance the received intensity on the detection system is therefore a critical issue. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification and genotyping in a single reaction. For the tests to the genotyping ability, the melting temperatures of B type HBV and C type HBV can be distinguished by the difference of 1.1degC.The test results in this research show the same degree of sensitivity for DNA quantification and reproducibility within five intra assay samples as compared with a commercial one
Starting Page	274
Ending Page	277
File Size	549417
Page Count	4
File Format	PDF
ISBN	1424401399
DOI	10.1109/NEMS.2006.334721
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-01-18
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Liver diseases Lab-on-a-chip Fluorescence DNA Optical polymers Optical sensors Testing Optical detectors Stimulated emission Optical losses HCV Real-time PCR HBV
Content Type	Text
Resource Type	Article

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