NDLI: Multi-color fluorescence enhancement from a photonics crystal surface

Content Provider	IEEE Xplore Digital Library
Author	Pokhriyal, A. Lu, M. Schulz, S. Huang, C.S. Cunningham, B.T.
Copyright Year	2010
Description	Author affiliation: SRU Biosystems, Woburn, USA (Lu, M.; Schulz, S.) \|\| Department of Physics, University of Illinois at Urbana- Champaign, USA (Pokhriyal, A.) \|\| Department of Electrical and Computer Engineering, University of Illinois at Urbana- Champaign, USA (Huang, C.S.; Cunningham, B.T.)
Abstract	A photonic crystal substrate exhibiting resonant enhancement of multiple fluorophores has been demonstrated. The device, fabricated uniformly from plastic materials over a ∼3×5 $in^{2}$ surface area by nanoreplica molding, utilizes two distinct resonant modes to enhance electric field stimulation of a dye excited by a λ = 632.8 nm laser (cyanine-5) and a dye excited by a λ = 532 nm laser (cyanine-3). Resonant coupling of the laser excitation to the photonic crystal surface is obtained for each wavelength at a distinct incident angle. Compared to detection of a dye-labeled protein on an ordinary glass surface, the photonic crystal surface exhibited a 32× increase in fluorescent signal intensity for cyanine-5 conjugated strepavidin labeling, while a 25× increase was obtained for cyanine-3 conjugated streptavidin labeling. The photonic crystal is capable of amplifying the output of any fluorescent dye with an excitation wavelength in the 532 nm < λ < 633 nm range by selection of an appropriate incident angle. The device is designed for biological assays that utilize multiple fluorescent dyes within a single imaged area, such as gene expression microarrays.
Starting Page	2287
Ending Page	2290
File Size	730332
Page Count	4
File Format	PDF
ISBN	9781424481705
ISSN	19300395
e-ISBN	9781424481699
e-ISBN	9781424481682
DOI	10.1109/ICSENS.2010.5690806
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-11-01
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence Optical surface waves Photonic crystals Surface waves Glass Laser excitation Surface treatment
Content Type	Text
Resource Type	Article
Subject	Electrical and Electronic Engineering

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