NDLI: Highly sensitive surface-enhanced raman nano-probing for direct proteomic profiling

Content Provider	IEEE Xplore Digital Library
Author	Yi Chen Zhida Xu Liu, L. Coppe, J.-P.
Copyright Year	2010
Description	Author affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, California 94720, USA (Coppe, J.-P.) \|\| Micro and Nanotechnology Laboratory, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, 61820, USA (Yi Chen; Zhida Xu; Liu, L.)
Abstract	Nanocone structures were fabricated by a unique etching-passivation process as the substrates for Surface Enhanced Raman Spectroscopy (SERS). This novel nanostructured SERS substrate allowed reaching unprecedented detection limits (fM of Rhodamin6G; pM of crude peptides), and supported the detection of subtle changes in peptide probe sequences, such as single amino acid phosphosphorylation or mutation. Thus, surface-conjugated peptides could be used as highly potent biological nano-sensors for sensitive profiling of specific protein-mediated chemical changes on nano-sensing probes. We hope such kinase-enzyme activity screening array device and technology will advance all aspects of Life Sciences, and potentially be applied to environment monitoring.
Starting Page	2295
Ending Page	2298
File Size	771735
Page Count	4
File Format	PDF
ISBN	9781424481705
ISSN	19300395
e-ISBN	9781424481699
e-ISBN	9781424481682
DOI	10.1109/ICSENS.2010.5690802
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-11-01
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Substrates Nanobioscience Probes Surface treatment Peptides Raman scattering Amino acids
Content Type	Text
Resource Type	Article
Subject	Electrical and Electronic Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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