NDLI: Discrimination of target proteins using arrayed fluorescent liposomes incorporated with cholesterol by principal component analysis

Content Provider	IEEE Xplore Digital Library
Author	Imamura, R. Zhang, Z. Yoshikawa, T. Murata, N. Yamashita, K. Fukuzawa, M. Noda, M. Shimanouchi, T.
Copyright Year	2015
Description	Author affiliation: Grad. Sch. of Environ. & Life Sci., Okayama Univ., Okayama, Japan (Shimanouchi, T.) \|\| Grad. Sch. of Sci. & Technol., Kyoto Inst. of Technol., Kyoto, Japan (Imamura, R.; Zhang, Z.; Yoshikawa, T.; Murata, N.; Yamashita, K.; Fukuzawa, M.; Noda, M.)
Abstract	A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were discriminated with high cumulative contribution ratio.
Sponsorship	IEEE
Starting Page	1
Ending Page	4
File Size	799902
Page Count	4
File Format	PDF
e-ISBN	9781479982035
DOI	10.1109/ICSENS.2015.7370493
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2015-11-01
Publisher Place	South Korea
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence Proteins Principal component analysis Arrays Biomembranes Lipidomics Optical filters Principal Component Analysis Biosensor Liposome Protein Array Cholesterol
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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