NDLI: A cell-based odor sensing system using fluorescent technique and lock-in measurement robust against disturbance

Content Provider	IEEE Xplore Digital Library
Author	Mujiono, T. Sukekawa, Y. Nakamoto, T. Mitsuno, H. Kanzaki, R. Misawa, N.
Copyright Year	2015
Description	Author affiliation: Precision & Intell. Lab., Tokyo Inst. of Technol., Tokyo, Japan (Mujiono, T.; Sukekawa, Y.; Nakamoto, T.) \|\| Res. Center for Adv. Sci. & Technol., Univ. of Tokyo, Tokyo, Japan (Mitsuno, H.; Kanzaki, R.) \|\| Electron.-Inspired Interdiscipl. Res. Inst., Toyohashi Univ. of Technol., Toyohashi, Japan (Misawa, N.)
Abstract	We have developed an odor sensing system based on insect olfactory receptor (OR) and fluorescent instrumentation. When an odorant is captured by an OR (OR56a) expressed on Sf21 cell, calcium indicator protein (GCaMP6) is activated by calcium ion flowing through the OR into the cell. The intensity change of the fluorescent light can be regarded as a sensor output. Since fluorescent light from the cell was very weak, the measurement should be done in the dark condition. The measurement quality deteriorates if the ambient light is included. Thus, a lock-in measurement technique was incorporated to the system to overcome the problem of the ambient light together with the fluorescent image. The lock-in technique enables the detection of light synchronous with the modulated excitation light under the ambient light. This technique is also useful to improve the signal to noise ratio. In this work, we were successful in the detection of 500 nM geosmin even under the condition with the ambient light, whereas it was not clearly detected by the system without lock-in technique.
Sponsorship	IEEE
Starting Page	1
Ending Page	4
File Size	1107576
Page Count	4
File Format	PDF
e-ISBN	9781479982035
DOI	10.1109/ICSENS.2015.7370351
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2015-11-01
Publisher Place	South Korea
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence Fluorescent lamps Frequency modulation Cameras Instruments Biosensors biosensor cell-based odor sensor fluorescent microscopy lock-in amplifier
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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