NDLI: Use of a gelatin cryogel as biomaterial scaffold in the differentiation process of human bone marrow stromal cells

Content Provider	IEEE Xplore Digital Library
Author	Fassina, L. Saino, E. Visai, L. Avanzini, M.A. Cusella De Angelis, M.G. Benazzo, F. Van Vlierberghe, S. Dubruel, P. Magenes, G.
Copyright Year	2010
Description	Author affiliation: University of Pavia, Dipartimento di Informatica e Sistemistica, C.I.T., Italy (Magenes, G.) \|\| University of Pavia, Dipartimento SMEC, IRCCS San Matteo, C.I.T., Italy (Benazzo, F.) \|\| University of Pavia, Dipartimento di Biochimica, C.I.T., Italy (Saino, E.; Visai, L.) \|\| IRCCS San Matteo of Pavia, Oncoematologia Pediatrica, Italy (Avanzini, M.A.) \|\| University of Pavia, Dipartimento di Medicina Sperimentale, C.I.T., Italy (Cusella De Angelis, M.G.) \|\| University of Ghent, Biomaterials Research Group, Belgium (Van Vlierberghe, S.; Dubruel, P.) \|\| University of Pavia, Dipartimento di Informatica e Sistemistica, Centro di Ingegneria Tissutale (C.I.T.), via Ferrata 1, 27100 Pavia, Italy (Fassina, L.)
Abstract	Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, gelatin cryogel scaffolds are promising new biomaterials owing to their biocompatibility; in addition, the in vitro modification of biomaterials with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering. In this study we have followed a biomimetic strategy where differentiated human bone marrow stromal cells built their extracellular matrix onto gelatin cryogel scaffolds. In comparison with control conditions without differentiation medium, the use of a differentiation medium increased, in vitro, the coating of gelatin cryogel with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The differentiation medium aimed at obtaining a better in vitro modification of gelatin cryogel in terms of cell colonization and coating with osteogenic signals, like bone matrix proteins. The modified biomaterial could be used, in clinical applications, as an implant for bone repair.
Starting Page	247
Ending Page	250
File Size	655266
Page Count	4
File Format	PDF
ISBN	9781424441235
ISSN	1557170X
DOI	10.1109/IEMBS.2010.5627475
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-08-31
Publisher Place	Argentina
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Bones Proteins In vitro Extracellular Immune system Humans
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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