NDLI: Microfabricated multi-electrode device for detecting oligodendrocyte-regulated changes in axonal conduction velocity

Content Provider	IEEE Xplore Digital Library
Author	Sakai, K. Shimba, K. Kotani, K. Jimbo, Y.
Copyright Year	2015
Description	Author affiliation: Res. Center for Adv. Sci. & Technol., Univ. of Tokyo, Tokyo, Japan (Kotani, K.) \|\| Sch. of Eng., Univ. of Tokyo, Tokyo, Japan (Sakai, K.; Jimbo, Y.) \|\| Grad. Sch. of Frontier Sci., Univ. of Tokyo, Tokyo, Japan (Shimba, K.)
Abstract	Myelin disorders cause cognitive dysfunction, but little is known about how abnormal myelin sheath affects neural activities at the network level. One reason for the lack is a technical difficulty in simultaneous monitoring of changes in both the axonal conduction and network activity. Then, we aimed to develop a culture device to detect myelination dependent changes in axonal conduction velocity in a neuronal network. The photolithographically fabricated device has microtunnels for guiding axons. Two microelectrodes and an oligodendrocyte (OL) culture compartment are set at each microtunnel. This configuration allows us to monitor changes in conduction velocity of axons wrapped by OLs. Neurons and OLs dissected from rat cortical tissues were cultured in the culture device. An immunocytochemical study indicated axonal growth and maturation of OL at 42 days in vitro (DIV), suggesting that neuron-OL co-culture was maintained in microtunnels. Propagating action potentials of individual axons were detected from spontaneous neural activities with a spike sorting method and their conduction velocities were examined. Conduction velocity without seeding OLs was 0.31 m/s, which was consistent with that of previous reports with unmyelinated axons. Although no apparent myelin sheath was observed in OL culture compartments, conduction delay with seeding OLs was approximately half as long as that without seeding OLs at 45 DIV. These results suggest that the culture device enables us to detect the OL-regulated changes in axonal conduction in the neuronal network.
Starting Page	7127
Ending Page	7130
File Size	834843
Page Count	4
File Format	PDF
ISSN	1557170X
e-ISBN	9781424492718
DOI	10.1109/EMBC.2015.7320035
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2015-08-25
Publisher Place	Italy
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Delays Electrodes Yttrium Biological neural networks Sorting
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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