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Content Provider | IEEE Xplore Digital Library |
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Author | Wong, C.Y. Mills, J.K. |
Copyright Year | 2015 |
Description | Author affiliation: Dept. of Mech. & Ind. Eng., Univ. of Toronto, Toronto, ON, Canada (Wong, C.Y.; Mills, J.K.) |
Abstract | Laser zona drilling (LZD), the ablation of a portion of the zona pellucida (ZP) in embryos with the use of a laser, is a required step in many embryonic surgical procedures such as assisted hatching and preimplantation genetic diagnosis. The objective of LZD is to remove specific locations of the ZP while minimizing potential harmful thermal effects to important structures of the embryo, namely the blastomeres. Current thermal analyzes of lasers used in LZD only encompass the use of a single pulse, whereas LZD is typically performed using multiple pulses. In this paper we analyze the effect of multipulse LZD and introduce a linear approximation method for multi-pulse LZD. Furthermore, we describe a novel method of measuring the thermal effect of a single laser pulse using the thermosensitive fluorescent dye Rhodamine B and a high speed camera. |
Starting Page | 2567 |
Ending Page | 2570 |
File Size | 2283666 |
Page Count | 4 |
File Format | |
ISSN | 1557170X |
e-ISBN | 9781424492718 |
DOI | 10.1109/EMBC.2015.7318916 |
Language | English |
Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Publisher Date | 2015-08-25 |
Publisher Place | Italy |
Access Restriction | Subscribed |
Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Subject Keyword | Embryo Laser beams Biological system modeling Temperature measurement Biopsy Temperature sensors Approximation methods |
Content Type | Text |
Resource Type | Article |
Subject | Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition |
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