Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Puts, R. Ambrosi, T. Kadow-Romacker, A. Raum, K. Ruschke, K. Knaus, P. |
| Copyright Year | 2014 |
| Description | Author affiliation: Berlin-Brandenburg Sch. for Regenerative Therapies, Julius Wolff Inst., Charité, Germany (Puts, R.; Ambrosi, T.; Kadow-Romacker, A.; Raum, K.) || Inst. for Chem. & Biochem., Free Univ., Berlin, Germany (Ruschke, K.; Knaus, P.) |
| Abstract | Low-Intensity Pulsed Ultrasound (LIPUS) is a safe treatment for fresh fractures, delayed- and non-union bone. Certain inconsistency is associated with the reported in-vitro data, which could be attributed to difficulties in reproducing similar conditions using unfocused transducers in experimental in-vitro set-ups. In our study we address the above issues by using focused LIPUS (FLIPUS), which avoids the previously described potential sources of physical side-effects and therefore, allows more robust “acoustic dose” definition and an “acoustic dose - observed effect” correlation in an in-vitro setting. In our experiments all relevant sound field parameters were measured and adjusted such that they provide an optimal and even sound transmission. Five days after daily FLIPUS stimulation, gene expression of osteopontin (OPN), Runx2, collagen-1 (Col-1), E11 antigen and dentin matrix protein-1 (DMP-1) was up-regulated in rat mesenchymal stem cells (rMSCs). These cells under reduced nutrient supply also showed higher proliferation rate on days 3 and 4. Enhanced proliferation on days 2, 3 and 4 was observed in murine MC3T3-E1 pre-osteoblasts after the FLIPUS treatment under similar conditions. Increased expressions of early response genes c-jun and cyclooxygenase-2 (COX-2) were observed 30 min post-FLIPUS-exposure in the same cells. FLIPUS induced enhanced phosphorylation of Smad1/5/8 (mothers against decapentaplegic) transcription factors in MC3T3-E1s, when Bone Morphogenetic Protein-2 (BMP-2) and FLIPUS were applied simultaneously. These results indicate that FLIPUS is a convenient set-up to study the mechanism of bone regeneration in response to ultrasound in vitro. |
| Starting Page | 1630 |
| Ending Page | 1633 |
| File Size | 684037 |
| Page Count | 4 |
| File Format | |
| e-ISBN | 9781479970490 |
| DOI | 10.1109/ULTSYM.2014.0404 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2014-09-03 |
| Publisher Place | USA |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Bones Ultrasonic imaging Acoustics Proteins Gene expression Media Transducers regeneration LIPUS acoustic dose BMP MSCs osteoblasts bone |
| Content Type | Text |
| Resource Type | Article |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|