NDLI: Specific detection of topoisomerase i from the malaria causing P. falciparum parasite using isothermal Rolling Circle Amplification

Content Provider	IEEE Xplore Digital Library
Author	Tesauro, C. Juul, S. Arno, B. Nielsen, C.J.F. Fiorani, P. Frohlich, R.F. Andersen, F.F. Desideri, A. Stougaard, M. Petersen, E. Knudsen, B.R.
Copyright Year	2012
Description	Author affiliation: Dept. of Biol., Univ. of Rome Tor Vergata, Rome, Italy (Tesauro, C.; Arno, B.; Fiorani, P.; Desideri, A.) \|\| Dept. of Pathology, Aarhus Univ. Hosp., Aarhus, Denmark (Stougaard, M.) \|\| Dept. of Clinical Med., Aarhus Univ. Hosp., Aarhus, Denmark (Petersen, E.) \|\| Dept. of Mol. Biol. & Genetics, Aarhus Univ., Aarhus, Denmark (Juul, S.; Nielsen, C.J.F.; Frohlich, R.F.; Andersen, F.F.; Knudsen, B.R.)
Abstract	We present a Rolling-Circle-Enhance-Enzyme-Activity-Detection (REEAD) system with potential use for future point-of-care diagnosis of malaria. In the developed setup, specific detection of malaria parasites in crude blood samples is facilitated by the conversion of single Plasmodium falciparum topoisomerase I (pfTopI) mediated cleavage-ligation events, happening within nanometer dimensions, to micrometer-sized products readily detectable at the single molecule level in a fluorescence microscope. In principle, REEAD requires no special equipment and the readout is adaptable to simple colorimetric detection systems. Moreover, with regard to detection limit the presented setup is likely to outcompete standard gold immuno-based diagnostics. Hence, we believe the presented assay forms the basis for a new generation of easy-to-use diagnostic tools suitable for the malaria epidemic areas in developing countries.
Sponsorship	IEEE Eng. Medicine Biol. Soc.
Starting Page	2416
Ending Page	2419
File Size	781614
Page Count	4
File Format	PDF
ISBN	9781424441198
ISSN	1557170X
e-ISBN	9781457717871
DOI	10.1109/EMBC.2012.6346451
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-08-28
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Substrates DNA Biochemistry Diseases Blood Humans Educational institutions
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Unsupervised malaria parasite detection based on phase spectrum

Optimized Detection of Plasmodium falciparum Topoisomerase I Enzyme Activity in a Complex Biological Sample by the Use of Molecular Beacons

Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy

Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level

Non-instrumented nucleic acid amplification (NINA): Instrument-free molecular malaria diagnostics for low-resource settings

Detection of malaria parasites in thick blood films

How do chondrocytes aggregate on fibroin substrate

Biomedical Engineering curriculum at UAM-I: A critical review

Rolling circle amplification as isothermal gene amplification in molecular diagnostics

Specific detection of topoisomerase i from the malaria causing P. falciparum parasite using isothermal Rolling Circle Amplification

Similar Documents

Unsupervised malaria parasite detection based on phase spectrum

Optimized Detection of Plasmodium falciparum Topoisomerase I Enzyme Activity in a Complex Biological Sample by the Use of Molecular Beacons

Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy

Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level

Non-instrumented nucleic acid amplification (NINA): Instrument-free molecular malaria diagnostics for low-resource settings

Detection of malaria parasites in thick blood films

How do chondrocytes aggregate on fibroin substrate

Biomedical Engineering curriculum at UAM-I: A critical review

Rolling circle amplification as isothermal gene amplification in molecular diagnostics

Specific detection of topoisomerase i from the malaria causing P. falciparum parasite using isothermal Rolling Circle Amplification