NDLI: Single camera system for multi-wavelength fruorescent imaging in the heart

Content Provider	IEEE Xplore Digital Library
Author	Yamanaka, T. Arafune, T. Shibata, N. Honjo, H. Kamiya, K. Kodama, I. Sakuma, I.
Copyright Year	2012
Description	Author affiliation: Shinjuku Mitsui Building Clinic, Tokyo, Japan (Shibata, N.) \|\| Dept. of Precision Eng., Univ. of Tokyo, Tokyo, Japan (Yamanaka, T.; Arafune, T.; Sakuma, I.) \|\| Res. Inst. of Environ. Med., Nagoya Univ., Nagoya, Japan (Honjo, H.; Kamiya, K.; Kodama, I.)
Abstract	Optical mapping has been a powerful method to measure the cardiac electrophysiological phenomenon such as membrane $potential(V_{m}),$ intracellular $calcium(Ca^{2+}),$ and the other electrophysiological parameters. To measure two parameters simultaneously, the dual mapping system using two cameras is often used. However, the method to measure more than three parameters does not exist. To exploit the full potential of fluorescence imaging, an innovative method to measure multiple, more than three parameters is needed. In this study, we present a new optical mapping system which records multiple parameters using a single camera. Our system consists of one camera, custom-made optical lens units, and a custom-made filter wheel. The optical lens units is designed to focus the fluorescence light at filter position, and form an image on camera's sensor. To obtain optical signals with high quality, efficiency of light collection was carefully discussed in designing the optical system. The developed optical system has object space numerical aperture(NA) 0.1, and image space NA 0.23. The filter wheel was rotated by a motor, which allows filter switching corresponding with needed fluorescence wavelength. The camera exposure and filter switching were synchronized by phase locked loop, which allow this system to record multiple fluorescent signals frame by frame alternately. To validate the performance of this system, we performed experiments to observe $V_{m}$ and $Ca^{2+}$ dynamics simultaneously (frame rate: 125fps) with Langendorff perfused rabbit heart. Firstly, we applied basic stimuli to the heart base (cycle length: 500ms), and observed planer wave. The waveforms of $V_{m}$ and $Ca^{2+}$ show the same upstroke synchronized with cycle length of pacing. In addition, we recorded $V_{m}$ and $Ca^{2+}$ signals during ventricular fibrillation induced by burst pacing. According to these experiments, we showed the efficacy and availability of our method for cardiac electrophysiological research.
Sponsorship	IEEE Eng. Medicine Biol. Soc.
Starting Page	3716
Ending Page	3719
File Size	834772
Page Count	4
File Format	PDF
ISBN	9781424441198
ISSN	1557170X
e-ISBN	9781457717871
DOI	10.1109/EMBC.2012.6346774
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-08-28
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Optical imaging Optical sensors Cameras Optical filters Optical recording Integrated optics Optical variables measurement
Content Type	Text
Resource Type	Article
Subject	Signal Processing Biomedical Engineering Health Informatics Computer Vision and Pattern Recognition

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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