NDLI: Systemic virus dissemination during local gene delivery in solid tumors and its control with an alginate solution

Content Provider	IEEE Xplore Digital Library
Author	Yong Wang Chuan-Yuan Li Fan Yuan
Copyright Year	2004
Description	Author affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA (Yong Wang)
Abstract	Intratumoral infusion, a routine method for local gene delivery in solid tumors, may cause a systemic dissemination of gene vectors. This is because tumor vessels are intrinsically leaky and intratumoral injection can also result in damage of tumor vessels. To this end, we investigated the extent of virus dissemination during and after local infusion of adenoviral vectors into a murine adenocarcinoma (4T1) transplanted in mice. Three different vectors were used in this study, they contained genes encoding either mouse interleukin-12 (IL-12), luciferase, or enhanced green fluorescence protein (EGFP). The virus distribution in the body was determined as the transgene expression in normal and tumor tissues. Our data demonstrated that a large fraction of injected viral vectors disseminated into liver tissues. To reduce the dissemination problem, we developed a novel method for viral vector delivery based on an alginate solution. We observed that this vehicle could significantly reduce virus dissemination without compromising the therapeutic efficacy of adenoviral vectors. Taken together, the data suggests that systemic virus dissemination is a serious problem in local gene therapy in tumors and that the dissemination can be significantly reduced by an alginate-based polymeric vehicle.
Starting Page	3524
Ending Page	3526
File Size	656105
Page Count	3
File Format	PDF
ISBN	0780384393
DOI	10.1109/IEMBS.2004.1403991
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2004-09-01
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Solids Control systems Mice Vehicles Encoding Fluorescence Proteins Liver neoplasms Gene therapy Polymers alginate virus dissemination gene delivery
Content Type	Text
Resource Type	Article

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