| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Zhou, Sujun Ge, Zhijun Feng, Jiake Ma, Tieliang Chen, Hong Lin, Jie Tan, Yongfei Ding, Weiliang Zhu, Wenjiao |
| Description |
Author Affiliation: Feng J ( Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Ma T ( Central Laboratory, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Ge Z ( Department of Critical Care Medicine, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Lin J ( Department of Cardiac and Thoracic Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Ding W ( Central Laboratory, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Chen H ( Department of Oncology, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Zhu W ( Central Laboratory, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Zhou S ( Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Tan Y ( Department of Cardiac and Thoracic Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.) |
| Abstract |
The aim of the current study was to investigate the effect of the PKM2 gene on the proliferation, invasion, migration and apoptosis of Panc1 and Sw1990 pancreatic cancer cells via its interaction with the mitogenactivated protein kinases (MAPKs) signaling pathways. The expression levels of PKM2 protein in pancreatic cancer cells and the corresponding normal tissues was determined with western blot analysis. Immunohistochemical analysis of PKM2 expression was carried out in paraffinembedded sections of pancreatic cancer tissue. Two human pancreatic cancer cell lines were cultured in vitro, and a small interfering RNA (siRNA) was designed for the PKM2 gene and transfected into the cells. Cell proliferation was measured via an MTT assay, cell migration and invasion was measured via Transwell® chambers, and the effect of PKM2 on apoptosis was detected from Bcell lymphoma 2 (Bcl2) and Bcl2associated X protein expression levels. Protein expression levels of the MAPK pathway proteins extracellular signalregulated kinase 1/2 (ERK1/2), p38 and cJun Nterminal kinase (JNK) and their phosphorylated forms were measured via western blot analysis. The expression level of PKM2 was significantly upregulated in the pancreatic cancer tissue compared with that of the corresponding normal tissue. Downregulation of PKM2 expression reduced the proliferation, migration and invasion of pancreatic cancer cell lines, while increasing the levels of apoptosis. Additionally, the expression levels of the phosphorylated(p)ERK1/2 and pp38 of the MAPK pathway in the PKM2 siRNA groups were markedly downregulated compared with those of the controls; however, the expression levels of ERK1/2, p38, JNK, pp38 and pJNK had no significantly changes compared with those of the control groups. In summary, the PKM2 gene has an important role in the proliferation, invasion, migration and apoptosis of Panc1 and Sw1990 pancreatic cancer cells, which may be associated with the expression of ERK1/2 and p38 of the MAPK signaling cascade. |
| ISSN |
17912997 |
| e-ISSN |
17913004 |
| Journal |
Molecular Medicine Reports |
| Issue Number |
3 |
| Volume Number |
11 |
| Language |
English |
| Publisher |
Spandidos Publications |
| Publisher Date |
2015-03-01 |
| Publisher Place |
Greece |
| Access Restriction |
Open |
| Subject Keyword |
Carrier Proteins Genetics Metabolism Map Kinase Signaling System Membrane Proteins Pancreatic Neoplasms Thyroid Hormones Apoptosis Cell Line, Tumor Cell Movement Cell Proliferation Gene Expression Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Rna, Small Interfering Research Support, Non-u.s. Gov't Discipline Molecular Biology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
