| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Luo, De-Yi Li, Hong Wang, Kun-Jie Dai, Yi Wazir, Romel Tian, Ye Yue, Xuan |
| Description |
Author Affiliation: Dai Y ( Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.); Tian Y ( Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.); Luo DY ( Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.); Wazir R ( Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.); Yue X ( Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.); Li H ( Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.); Wang KJ ( Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.) |
| Abstract |
The present study aimed to investigate whether the cyclic stretchinduced proliferation of human bladder smooth muscle cells (HBSMCs) is mediated by muscarinic (M) receptors, together with the signal transduction mechanisms involved in this process. HBSMCs seeded onto silicone membranes were subjected to different cyclic stretches (5, 10, 15 and 20%) for 6 and 12 h. As the effect of cyclic stretch on M2 and M3 mRNA expression levels was maximal at 6 h 10% stretch, all subsequent experiments were performed at this stretch. Western blot analysis was used to quantify M2, M3, protein kinase C (PKC) and phosphorylated (p)PKC protein expression levels, flow cytometry was employed to examine cell cycle distribution and a 5-bromo2-deoxyuridine (BrdU) incorporation assay was used to assess cell proliferation at this stretch. Subsequently, HBSMCs were exposed to different acetylcholine concentrations and/or cyclic stretch, M receptor antagonists [AF-DX16, an M2 receptor antagonist; 1,1-dimethyl-4-diphenylacetoxypiperidinium iodide (4-DAMP), an M3 receptor antagonist and atropine, a nonselective antagonist] and GF 109203X, a PKC antagonist, to assess the possible underlying signaling mechanisms. Cyclic stretch was found to increase the proliferation of HBSMCs and the expression levels of M2, M3, PKC and pPKC proteins. M receptor and PKC antagonists exerted no apparent effect on nonstretched cells, but reduced the incorporation of BrdU into stretched cells; the most pronounced effects were observed when nonselective M receptor and PKC antagonists were applied. Notably, 4DAMP did not inhibit stretchinduced PKC activation. These results indicate that the activation of the M3 receptor signaling pathway in stretchinduced HBSMC proliferation occurs via PKC-independent mechanisms. |
| ISSN |
17912997 |
| e-ISSN |
17913004 |
| Journal |
Molecular Medicine Reports |
| Issue Number |
3 |
| Volume Number |
11 |
| Language |
English |
| Publisher |
Spandidos Publications |
| Publisher Date |
2015-03-01 |
| Publisher Place |
Greece |
| Access Restriction |
Open |
| Subject Keyword |
Myocytes, Smooth Muscle Metabolism Receptors, Muscarinic Stress, Mechanical Urinary Bladder Acetylcholine Pharmacology Cell Cycle Genetics Cell Proliferation Drug Effects Cells, Cultured Enzyme Activation Gene Expression Muscarinic Antagonists Protein Kinase C Antagonists & Inhibitors Rna, Messenger Signal Transduction Research Support, Non-u.s. Gov't Discipline Molecular Biology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
