| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Ge, Zhijun Chen, Yanyu Feng, Jiake Ma, Tieliang Chen, Hong Tang, Xiaoyan Tan, Yongfei Ding, Weiliang Chen, Xiang Zhu, Wenjiao |
| Description |
Author Affiliation: Chen X ( Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Feng J ( Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Ge Z ( Department of Critical Care Medicine, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Chen H ( Department of Oncology, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Ding W ( Central Laboratory, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Zhu W ( Central Laboratory, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Tang X ( Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Chen Y ( Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Tan Y ( Department of Cardiac and Thoracic Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.); Ma T ( Central Laboratory, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.) |
| Abstract |
The present study aimed to investigate whether the JWA gene can regulate the proliferation, migration and invasion of human breast cancer cells through the MAPK signaling pathway. The role of JWA in proliferation, migration, invasion and apoptosis was investigated in the MDAMB231 human breast cancer cell line. Following transfection with JWAsmall interfering (si)RNA, the effect of JWA on apoptosis was assessed by Western blot analysis, proliferation was determined using Transwell chambers and cell migration and invasion were analyzed by transwell assay. The expression levels of extracellular signalregulated kinase (ERK) 1/2, CSBP/RK/Mpk2 kinase (p38) and cJun Nterminal kinase (JNK) were detected using Western blot analysis in the siRNA and control groups. The expression of JWA in the breast cancer cells was significantly lower compared with the normal breast cells. Downregulation of JWA protein levels reduced the apoptosis and enhanced proliferation, migration and invasion of the MDAMB231 cells in vitro. The results of the Western blot analysis demonstrated that, compared with the control groups, the expression levels of phosphorylated (p)p38 decreased significantly in the JWA siRNA group. No significant changes were observed in the expression levels of pERK1/2 or pJNK. Therefore, the JWA gene may regulate human breast cancer cells through the MAPK signaling pathway using different types of regulation. |
| ISSN |
17912997 |
| e-ISSN |
17913004 |
| Journal |
Molecular Medicine Reports |
| Issue Number |
5 |
| Volume Number |
11 |
| Language |
English |
| Publisher |
Spandidos Publications |
| Publisher Date |
2015-05-01 |
| Publisher Place |
Greece |
| Access Restriction |
Open |
| Subject Keyword |
Breast Neoplasms Genetics Gene Expression Regulation, Neoplastic Heat-shock Proteins Intracellular Signaling Peptides And Proteins Apoptosis Metabolism Cell Line, Tumor Cell Movement Cell Proliferation Down-regulation Gene Knockdown Techniques Map Kinase Signaling System Mammary Glands, Human Rna, Small Interfering Research Support, Non-u.s. Gov't Discipline Molecular Biology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
