| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Wu, Shijie Li, Zhaoguo Kang, Xiaowen Zhang, Wei Xiao, Jinling Wu, Xiaomei Huang, Kun Wang, Xinyan |
| Description |
Author Affiliation: Huang K ( Department of Respiratory Medicine, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.); Kang X ( Department of Respiratory Medicine, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.); Wang X ( Department of Respiratory Medicine, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.); Wu S ( Department of Respiratory Medicine, Daqing Oilfield General Hospital, Daqing, Heilongjiang 163316, P.R. China.); Xiao J ( Department of Respiratory Medicine, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.); Li Z ( Department of Respiratory Medicine, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.); Wu X ( Department of Respiratory Medicine, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.); Zhang W ( Department of Respiratory Medicine, The First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.) |
| Abstract |
Pulmonary fibrosis is an irreversible chronic progressive fibroproliferative lung disease, which usually has a poor prognosis. Previous studies have confirmed that the transplantation of bone marrow mesenchymal stem cells (MSCs) significantly reduces lung damage in a number of animal models. However, the underlying mechanism involved in this process remains to be elucidated. In the present study, a bleomycin (BLM)induced female Wister rat model of fibrosis was established. At 0 or 7 days following BLM administration, rats were injected into the tail vein with 5bromo2deoxyuridinelabeled MSCs extracted from male Wistar rats. The lung tissue of the rats injected with MSCs expressed the sexdetermining region Y gene. The level surfactant protein C (SPC), a marker for type II alveolar epithelial cells (AEC II), was higher in the group injected with MSCs at day 0 than that in the group injected at day 7. Furthermore, SPC mRNA, but not aquaporin 5 mRNA, a marker for type I alveolar epithelial cells, was expressed in fresh bone marrow aspirates and the fifth generation of cultured MSCs. In addition, superoxide dismutase activity and total antioxidative capability, specific indicators of oxidative stress, were significantly increased in the lung tissue of the MSCtransplanted rats (P<0.05). In conclusion, to alleviate pulmonary fibrosis, exogenous MSCs may be transplanted into damaged lung tissue where they differentiate into AEC II and exert their effect, at least in part, through blocking oxidative stress. |
| ISSN |
17912997 |
| e-ISSN |
17913004 |
| DOI |
10.3892/mmr.2014.2981 |
| Journal |
Molecular Medicine Reports |
| Issue Number |
3 |
| Volume Number |
11 |
| Language |
English |
| Publisher |
Spandidos Publications |
| Publisher Date |
2015-03-01 |
| Publisher Place |
Greece |
| Access Restriction |
Open |
| Subject Keyword |
Pneumocytes Cytology Cell Transdifferentiation Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells Oxidative Stress Pulmonary Fibrosis Metabolism Animals Aquaporin 5 Genetics Disease Models, Animal Gene Expression Pathology Therapy Pulmonary Surfactant-associated Protein C Rna, Messenger Research Support, Non-u.s. Gov't Discipline Molecular Biology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology |
