NDLI: Reduced tumorigenicity and drug resistance through the downregulation of octamer-binding protein 4 and Nanog transcriptional factor expression in human breast stem cells.

Please wait, while we are loading the content...

Year: 2017 Volume: 15

Year: 2016 Volume: 14

Year: 2016 Volume: 13

Year: 2015 Volume: 12

Year: 2015 Volume: 11

Year: 2015 Volume: 11 Issue: 6

Year: 2015 Volume: 11 Issue: 5

Year: 2015 Volume: 11 Issue: 4

Year: 2015 Volume: 11 Issue: 3

Baicalein induces apoptosis of human cervical cancer HeLa cells in vitro.

Differential microRNA expression profile comparison between epidermal stem cells and differentiated keratinocytes.

Treatment with Lingqi capsules suppresses colorectal cancer by inhibiting the hepatocyte growth factor/cMet signal transduction pathway.

Roles of glucose transporter-1 and the phosphatidylinositol 3kinase/protein kinase B pathway in cancer radioresistance (review).

Cyclindependent kinase inhibitor p21 does not impact embryonic endochondral ossification in mice.

Differential expression of microRNAs and their target genes in non-small-cell lung cancer.

Dihydromyricetin induces mouse hepatoma Hepal-6 cell apoptosis via the transforming growth factor-ß pathway.

Isolation and characterization of ex vivo expanded mesenchymal stem cells obtained from a surgical patient.

Effects of Treg cells and IDO on human epithelial ovarian cancer cells under hypoxic conditions.

Mutation analysis of Leber congenital amaurosisassociated genes in patients with retinitis pigmentosa.

Optimizing methods for the study of intravascular lipid metabolism in zebrafish.

A preliminary study on the role of the complement regulatory protein, cluster of differentiation 55, in mice with diabetic neuropathic pain.

Hepatitis C virus represses the cellular antiviral response by upregulating the expression of signal transducer and activator of transcription 3 through sponging microRNA122.

Stress exposure and postural control in young females.

Protective role of Klotho on cardiomyocytes upon hypoxia/reoxygenation via downregulation of Akt and FOXO1 phosphorylation.

Axis inhibition protein 2 polymorphisms may be a risk factor for families with isolated oligodontia.

GYY4137, a hydrogen sulfidereleasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factorkappa B and mitogenactivated protein kinases in Coxsackie virus B3infected rat cardiomyocytes.

Roscovitine-treated HeLa cells finalize autophagy later than apoptosis by downregulating Bcl2.

Inhibition of osteosarcoma cell progression by MacroH2A via the downregulation of cyclin D and cyclindependent kinase genes.

Twist-related protein 1 enhances oral tongue squamous cell carcinoma cell invasion through ß-catenin signaling.

Antitumor activity of adenoviral vector containing T42 and 4xT42 peptide gene through inducing apoptosis of tumor cells and suppressing angiogenesis.

Increased monocytic CD14â ºHLADRlow/- myeloid-derived suppressor cells in obesity.

Mild clinical manifestation and unusual recovery upon coenzyme Q10 treatment in the first Chinese Leigh syndrome pedigree with mutation m.10197 G>A.

Downregulation of gangliotetraosylceramide and ß1,3-galactosyltransferase-4 gene expression by Smads during transforming growth factor ß-induced epithelial-mesenchymal transition.

Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1 protein.

Ki67 is a promising molecular target in the diagnosis of cancer (review).

Algal sulfated carrageenan inhibits proliferation of MDA-MB-231 cells via apoptosis regulatory genes.

Soluble P-selectin promotes acute myocardial infarction onset but not severity.

Radiation-induced matrix metalloproteinases limit natural killer cell-mediated anticancer immunity in NCI-H23 lung cancer cells.

Suppression of dendritic cell and T-cell activation by the pRST98 Salmonella plasmid.

When Cri du chat syndrome meets Edwards syndrome.

Effect of ulinastatin on the expression and distribution of high mobility group box 1 in human colon carcinoma cells in vitro.

Effects of hypothermia combined with neural stem cell transplantation on recovery of neurological function in rats with spinal cord injury.

Expression and functional role of Nav1.9 sodium channel in cartwheel cells of the dorsal cochlear nucleus.

Annexin A2 knockdown inhibits hepatoma cell growth and sensitizes hepatoma cells to 5-fluorouracil by regulating ß-catenin and cyclin D1 expression.

Transforming growth factor-ß1 small interfering RNA inhibits growth of human embryonic lung fibroblast HFL-I cells in vitro and defends against radiation-induced lung injury in vivo.

Propofol alleviates acute lung injury following orthotopic autologous liver transplantation in rats via inhibition of the NADPH oxidase pathway.

Potential therapeutic mechanism of genistein in breast cancer involves inhibition of cell cycle regulation.

Resveratrol exerts an anti-apoptotic effect on human bronchial epithelial cells undergoing cigarette smoke exposure.

Association of the paired box 2 gene polymorphism with the susceptibility and pathogenesis of HenochSchönlein purpura in children.

Effects of 5fluorouracil and class III phosphoinositide 3kinase small interfering RNA combination therapy on SGC7901 human gastric cancer cells.

An insight into the key genes and biological functions associated with insulin resistance in adipose tissue with microarray technology.

Reversing effect and mechanism of soluble resistance-related calcium-binding protein on multidrug resistance in human lung cancer A549/DDP cells.

Omentin-1 promotes the growth of neural stem cells via activation of Akt signaling.

Synthesis, characterization and in vitro and in vivo investigation of C3F8-filled poly(lactic-co-glycolic acid) nanoparticles as an ultrasound contrast agent.

Heat shock protein 72 suppresses apoptosis by increasing the stability of X-linked inhibitor of apoptosis protein in renal ischemia/reperfusion injury

Docosahexanoic acid modifies low-density lipoprotein receptor abundance in HepG2 cells via suppression of the LXR -Idol pathway.

Calcium ions promote primary renal epithelial cell differentiation into cells with bone-associated phenotypes via transforming growth factor-ß1-induced epithelial-mesenchymal transition in idiopathic hypercalciuria patients.

Protective effect of carboxymethylated chitosan on hydrogen peroxide-induced apoptosis in nucleus pulposus cells

Effects of branched-chain amino acids and zinc-enriched nutrients on prognosticators in HCV-infected patients: a multicenter randomized controlled trial.

Effects of mild induced hypothermia on hippocampal connexin 43 and glutamate transporter 1 expression following traumatic brain injury in rats.

Detection of long-chain non-encoding RNA differential expression in non-small cell lung cancer by microarray analysis and preliminary verification.

Berberine targets epidermal growth factor receptor signaling to suppress prostate cancer proliferation in vitro.

Glycolysis inhibitor 2-deoxy-D-glucose suppresses carcinogen-induced rat hepatocarcinogenesis by restricting cancer cell metabolism.

Selective colorectal cancer cell lysates enhance the immune function of mature dendritic cells in vitro.

Inhibition of the signal transducer and activator of transcription 3 signaling pathway by Qianliening capsules suppresses the growth and induces the apoptosis of human prostate cells.

mRNA expression of glucocorticoid receptor and serological and virological markers of chronic hepatitis B.

Adiponectin reduces carotid atherosclerotic plaque formation in ApoE-/- mice: roles of oxidative and nitrosative stress and inducible nitric oxide synthase.

Expression patterns of dishevelled-2 in different colon tissue segments in Hirschsprung's disease.

Downregulation of c-Met expression does not enhance the sensitivity of gastric cancer cell line MKN-45 to gefitinib.

Overexpression of activated leukocute cell adhesion molecule in gastric cancer is associated with advanced stages and poor prognosis and miR-9 deregulation.

Upregulation of Stat1-HDAC4 confers resistance to etoposide through enhanced multidrug resistance 1 expression in human A549 lung cancer cells.

Pre-treatment with a Xingnaojing preparation ameliorates sevoflurane-induced neuroapoptosis in the infant rat striatum.

Adipose tissue-derived mesenchymal stem cells differentiated into hepatocyte-like cells in vivo and in vitro.

Characteristics of stem cells derived from rat fascia: in vitro proliferative and multilineage potential assessment.

Apigenin inhibits the self-renewal capacity of human ovarian cancer SKOV3derived sphere-forming cells.

Picosecond pulsed electric fields induce apoptosis in a cervical cancer xenograft.

Endoplasmic reticulum stress preconditioning antagonizes low-density lipoprotein-induced inflammation in human mesangial cells through upregulation of XBP1 and suppression of the IRE1 /IKK/NF-κB pathway.

Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells.

Cardioprotective effects of tilianin in rat myocardial ischemia-reperfusion injury.

Nitric oxide synthases, S-nitrosylation and cardiovascular health: from molecular mechanisms to therapeutic opportunities (review).

Balance between metallothionein and metal response element binding transcription factor 1 is mediated by zinc ions (review).

Effects of modified Shu-Gan-Liang-Xue decoction combined with anastrozole on osteoblastic proliferation and differentiation of MC3T3-E1 cells.

Domain I-IV of ß2-glycoprotein I inhibits advanced glycation end product-induced angiogenesis by down-regulating vascular endothelial growth factor 2 signaling.

Endoplasmic reticulum stress protects human thyroid carcinoma cell lines against ionizing radiation-induced apoptosis.

Rab25 is responsible for phosphoinositide 3-kinase/AKTmediated cisplatin resistance in human epithelial ovarian cancer cells.

Mouse dead end 1-ß interacts with c-Jun and stimulates activator protein 1 transactivation.

MicroRNA let-7a suppresses the growth and invasion of cholesteatoma keratinocytes.

MicroRNA-451a is associated with cell proliferation, migration and apoptosis in renal cell carcinoma.

Effects of long-term soft contact lens wear on the corneal thickness and corneal epithelial thickness of myopic subjects.

Downregulation of osteopontin enhances the sensitivity of glioma U251 cells to temozolomide and cisplatin by targeting the NF-κB/Bcl2 pathway.

Reduced tumorigenicity and drug resistance through the downregulation of octamer-binding protein 4 and Nanog transcriptional factor expression in human breast stem cells.

Curcumin-induced histone acetylation inhibition improves stress-induced gastric ulcer disease in rats.

Effect of Melilotus extract on lung injury via the upregulation of tumor necrosis factor- -induced protein-8-like 2 in septic mice.

Impact of losartan and angiotensin II on the expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in rat vascular smooth muscle cells.

Identification of bile biomarkers of biliary tract cancer through a liquid chromatography/mass spectrometry-based metabolomic method.

Bone marrow-derived mesenchymal stem cells protect against lung injury in a mouse model of bronchopulmonary dysplasia.

Effects of sevoflurane on leucine-rich repeat kinase 2-associated Drosophila model of Parkinson's disease.

Expression of B-cell-associated genes in peripheral blood mononuclear cells of patients with symptomatic pulmonary embolism.

Novel oncolytic adenovirus sensitizes renal cell carcinoma cells to radiotherapy via mitochondrial apoptotic cell death.

Reduction of isoproterenol-induced cardiac hypertrophy and modulation of myocardial connexin43 by a KATP channel agonist.

Para-phenylenediamine-induces apoptosis via a pathway dependent on PTK-Ras-Raf-JNK activation but independent of the PI3K/Akt pathway in NRK-52E cells.

Effect of cyclin-dependent kinase 7 silencing on cisplatin sensitivity in endometrial carcinoma cells.

Aberrantly expressed mRNAs and long non-coding RNAs in patients with invasive ductal breast carcinoma: a pilot study.

Conversion of bone marrow mesenchymal stem cells into type II alveolar epithelial cells reduces pulmonary fibrosis by decreasing oxidative stress in rats.

PKM2 gene regulates the behavior of pancreatic cancer cells via mitogen-activated protein kinase pathways.

Lutein protects against ischemia/reperfusion injury in rat kidneys.

Blockade of 11ß-hydroxysteroid dehydrogenase type 1 enzyme inhibits experimental collagenase-induced osteoarthritis.

Cyclic stretch induces human bladder smooth muscle cell proliferation in vitro through muscarinic receptors.

Proliferative response of peripheral blood mononuclear cells in anti-Hu antibody-associated patients with paraneoplastic neurological syndrome and their depressant effect on small cell lung cancer cells.

Umbilical cord-derived mesenchymal stem cell therapy for neurological disorders via inhibition of mitogen-activated protein kinase pathway-mediated apoptosis.

Simvastatin induces growth inhibition and apoptosis in HepG2 and Huh7 hepatocellular carcinoma cells via upregulation of Notch1 expression.

Localization and expression of heat shock protein 70 with rat myocardial cell damage induced by heat stress in vitro and in vivo.

Inhibition of poly (ADP-ribose) polymerase and inducible nitric oxide synthase protects against ischemic myocardial damage by reduction of apoptosis.

5-aminolevulinic acid-induced protoporphyrin IX with multi-dose ionizing irradiation enhances host antitumor response and strongly inhibits tumor growth in experimental glioma in vivo.

Octamer-binding protein 4 affects the cell biology and phenotypic transition of lung cancer cells involving ß-catenin/E-cadherin complex degradation.

Potential roles of the RGMa-FAK-Ras pathway in hippocampal mossy fiber sprouting in the pentylenetetrazole kindling model.

MicroRNA-155 promotes the proliferation and invasion abilities of colon cancer cells by targeting quaking.

Reduction of endotoxin attenuates liver fibrosis through suppression of hepatic stellate cell activation and remission of intestinal permeability in a rat non-alcoholic steatohepatitis model.

Capsinoids suppress fat accumulation via lipid metabolism.

Regulatory network of differentially expressed genes in metastatic osteosarcoma.

Proteasome inhibitor-induced autophagy in PC12 cells overexpressing A53T mutant α-synuclein

Protective effects of tetrandrine on brain cells in phenobarbital-dependent and -withdrawn rats.

Antitumor and apoptotic activities of the chemical constituents from the ethyl acetate extract of Artemisia indica.

Effect of fibroblast growth factor 9 on the osteogenic differentiation of bone marrow stromal stem cells and dental pulp stem cells.

Year: 2015 Volume: 11 Issue: 2

Year: 2015 Volume: 11 Issue: 1

Year: 2014 Volume: 10

Year: 2014 Volume: 9

Year: 2013 Volume: 8

Year: 2013 Volume: 7

Year: 2012 Volume: 6

Year: 2012 Volume: 5

Year: 2011 Volume: 4

Year: 2010 Volume: 3

Year: 2008 Volume: 1

Reduced tumorigenicity and drug resistance through the downregulation of octamer-binding protein 4 and Nanog transcriptional factor expression in human breast stem cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wu, Bing-Lin Jiang, Long Luo, Qi Feng, Qing-Zhao Huang, Zheng-Jie You, Jun Luo, Wei-Yuan Chen, Bai-Sheng
Description	Author Affiliation: Huang ZJ ( Department of Surgical Oncology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.); You J ( Department of Surgical Oncology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.); Luo WY ( Department of Surgical Oncology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.); Chen BS ( Department of Surgical Oncology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.); Feng QZ ( Department of Surgical Oncology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.); Wu BL ( Department of Surgical Oncology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.); Jiang L ( Department of Surgical Oncology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.); Luo Q ( Department of Surgical Oncology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.)
Abstract	Breast cancer is the most common type of malignancy among females. Previous studies examining breast cancer tissue have demonstrated the presence of stem cells, and have detected octamerbinding protein 4 (Oct4) and Nanog transcription factor expression. In the present study, breast cancer stem cells (CSCs) were isolated and enriched from MDAMB231 breast cancer cell lines, and were defined as MDAMB231 stem cells using flow cytometry. The expression of Oct4 and Nanog in breast CSCs were detected by quantitative polymerase chain reaction and western blotting. RNA interference (RNAi) was used in order to downregulate the expression of Oct4 and Nanog. Drug resistance and tumorinitiating capability following in vivo injection of MDAMB231 stem cells trans-duced with negative RNAi, Oct4 RNAi and Nanog RNAi were compared with that of MDAMB231 stem cells without siRNA transfection as a control group. In addition the capability of MDAMB231 breast cancer cells to initiate tumor formation in mice was compared with that of MDAMB231 stem cells. A paclitaxel inhibition test was also conducted in order to detect resistance of MDAMB231 breast cancer stem cells to this treatment. The MDAMB231 stem cells were revealed to exhibit elevated percentages of the cluster of differentiation (CD)44+CD24/low subset, high tumorigenicity and resistance to chemotherapy, all of which are characteristic stem cell properties. In addition, the MDAMB231 stem cells were more tumorigenic in vivo. Furthermore, the breast CSCs also expressed high levels of the Oct4 and Nanog transcription factors. Therefore, downregulation of Oct4 or Nanog expression may reduce chemotherapeutic drug resistance and tumorigenicity in breast CSCs. In conclusion, Oct4 and Nanog expression may be a key factor in the development of resistance to chemotherapy and tumor growth of breast CSCs. This finding indicates that Oct4 or Nanogtargeted therapy may be a promising means of overcoming resistance to chemotherapy and inhibiting tumor growth in breast cancer treatment.
ISSN	17912997
e-ISSN	17913004
DOI	10.3892/mmr.2014.2972
Journal	Molecular Medicine Reports
Issue Number	3
Volume Number	11
Language	English
Publisher	Spandidos Publications
Publisher Date	2015-03-01
Publisher Place	Greece
Access Restriction	Open
Subject Keyword	Breast Neoplasms Genetics Cell Transformation, Neoplastic Drug Resistance Homeodomain Proteins Neoplastic Stem Cells Metabolism Octamer Transcription Factor-3 Antineoplastic Agents Pharmacology Pathology Cell Line, Tumor Down-regulation Gene Expression Paclitaxel Phenotype Spheroids, Cellular Tumor Cells, Cultured Research Support, Non-u.s. Gov't Discipline Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in