NDLI: Sclera as a surrogate marker for determining AGE-modifications in Bruch's membrane using a Raman spectroscopy-based index of aging.

Please wait, while we are loading the content...

Year: 2017 Volume: 58

Year: 2016 Volume: 57

Year: 2015 Volume: 56

Year: 2014 Volume: 56

Year: 2014 Volume: 55

Year: 2013 Volume: 54

Year: 2012 Volume: 53

Year: 2011 Volume: 52

Year: 2011 Volume: 52 Issue: 13

Year: 2011 Volume: 52 Issue: 12

Year: 2011 Volume: 52 Issue: 11

Year: 2011 Volume: 52 Issue: 10

Year: 2011 Volume: 52 Issue: 9

Year: 2011 Volume: 52 Issue: 8

Year: 2011 Volume: 52 Issue: 7

Year: 2011 Volume: 52 Issue: 6

Year: 2011 Volume: 52 Issue: 5

Year: 2011 Volume: 52 Issue: 4

Year: 2011 Volume: 52 Issue: 3

Induction of arginase II mRNA by nitric oxide using an in vitro model of gyrate atrophy of choroid and retina.

A survey of DNA variation of C2ORF71 in probands with progressive autosomal recessive retinal degeneration and controls.

Effect of disease severity and optic disc size on diagnostic accuracy of RTVue spectral domain optical coherence tomograph in glaucoma.

Oral administration of retinoic acid receptor-alpha/beta-specific ligand Am80 suppresses experimental autoimmune uveoretinitis.

Proteomic analyses of corneal tissue subjected to alkali exposure.

Molecular and clinical characterization of albinism in a large cohort of Italian patients.

Light-induced retinal degeneration correlates with changes in iron metabolism gene expression, ferritin level, and aging.

Potential novel mechanism for Axenfeld-Rieger syndrome: deletion of a distant region containing regulatory elements of PITX2.

IOP-induced lamina cribrosa displacement and scleral canal expansion: an analysis of factor interactions using parameterized eye-specific models.

Upregulation of mouse genes in HSV-1 latent TG after butyrate treatment implicates the multiple roles of the LAT-ICP0 locus.

In vivo ocular efficacy profile of mapracorat, a novel selective glucocorticoid receptor agonist, in rabbit models of ocular disease.

Investigating unstable fixation in patients with macular disease.

A role of corneal mechanical adaptation in contact lens-related dry eye symptoms.

A novel method for the induction of experimental glaucoma using magnetic microspheres.

XIAP therapy increases survival of transplanted rod precursors in a degenerating host retina.

Visual activity before and after the onset of juvenile myopia.

Human orbital muscle: a new point of view from the fetal development of extraocular connective tissues.

Factors impacting corneal epithelial barrier function against Pseudomonas aeruginosa traversal.

Surfactant properties of human meibomian lipids.

Correlation between refractive error, corneal power, and thickness in a large population with a wide range of ametropia.

Regulation of corneal epithelial barrier function by Kruppel-like transcription factor 4.

The influence of lamellar orientation on corneal material behavior: biomechanical and structural changes in an avian corneal disorder.

Evaluation of the comparability and repeatability of four wavefront aberrometers.

Differential expression of neuronal genes in Müller glia in two- and three-dimensional cultures.

Zebrafish Tg(7.2mab21l2:EGFP)ucd2 transgenics reveal a unique population of retinal amacrine cells.

Effect of contact lens power on optical coherence tomography of the retinal nerve fiber layer.

Interaction of arrestin with enolase1 in photoreceptors.

Early gene expression changes in the retinal ganglion cell layer of a rat glaucoma model.

Blood contrast agents enhance intrinsic signals in the retina: evidence for an underlying blood volume component.

The roles of vitreal macrophages and circulating leukocytes in retinal neovascularization.

Epigenetics, microRNAs, and carcinogenesis: functional role of microRNA-137 in uveal melanoma.

Validity of a new computer-aided diagnosis imaging program to quantify nuclear cataract from slit-lamp photographs.

Internal limiting membrane peeling versus no peeling for idiopathic full-thickness macular hole: a pragmatic randomized controlled trial.

Quantification of the therapeutic response of intraretinal, subretinal, and subpigment epithelial compartments in exudative AMD during anti-VEGF therapy.

Morphometric characteristics of central retinal artery and vein endothelium in the normal human optic nerve head.

Fixation stability during binocular viewing in patients with age-related macular degeneration.

Visual acuity development of children with infantile nystagmus syndrome.

Assessment of the contribution of cytokine plasma levels to detect retinopathy of prematurity in very low birth weight infants.

Immunohistochemical evidence of synaptic retraction, cytoarchitectural remodeling, and cell death in the inner retina of the rat model of oygen-induced retinopathy (OIR).

Revealing Henle's fiber layer using spectral domain optical coherence tomography.

The effect of ageing on in vivo human ciliary muscle morphology and contractility.

Cone structure in retinal degeneration associated with mutations in the peripherin/RDS gene.

Integrin-Linked Kinase Regulates Integrin Signaling in Human Trabecular Meshwork Cells

In vitro interactions of Fusarium and Acanthamoeba with drying residues of multipurpose contact lens solutions.

Total corneal power estimation: ray tracing method versus gaussian optics formula.

Differentially expressed genes associated with human limbal epithelial phenotypes: new molecules that potentially facilitate selection of stem cell-enriched populations.

Activation of P2X Receptors Induces Apoptosis in Human Retinal Pigment Epithelium

Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium

Effect of elevated intracellular cAMP levels on actomyosin contraction in bovine trabecular meshwork cells.

Age-related gene response of human corneal endothelium to oxidative stress and DNA damage.

Figure ground discrimination in age-related macular degeneration.

Polarized secretion of PEDF from human embryonic stem cell-derived RPE promotes retinal progenitor cell survival.

Three-dimensional imaging of macular inner structures in glaucoma by using spectral-domain optical coherence tomography.

Low Levels of Hydrogen Peroxide Stimulate Corneal Epithelial Cell Adhesion, Migration, and Wound Healing

Transcriptional regulation of activating transcription factor 4 under oxidative stress in retinal pigment epithelial ARPE-19/HPV-16 cells.

The use of mucoadhesive polymers to enhance the hypotensive effect of a melatonin analogue, 5-MCA-NAT, in rabbit eyes.

In vivo efficacy of bone marrow stromal cells coated with beta-tricalcium phosphate for the reconstruction of orbital defects in canines.

Non-damaging retinal phototherapy: dynamic range of heat shock protein expression.

Relationship of central corneal thickness with optic disc parameters: the Singapore Malay Eye Study.

Prophylactic effect of intravenous moxifloxacin in a rabbit model of Staphylococcus epidermidis endophthalmitis.

Dissection of chromosome 16p12 linkage peak suggests a possible role for CACNG3 variants in age-related macular degeneration susceptibility.

The effect of graded cyclic stretching on extracellular matrix-related gene expression profiles in cultured primary human lamina cribrosa cells.

Outflow physiology of the mouse eye: pressure dependence and washout.

The Impact of Vision Impairment for Children (IVI_C): validation of a vision-specific pediatric quality-of-life questionnaire using Rasch analysis.

Therapeutic effect of stealth-type polymeric nanoparticles with encapsulated betamethasone phosphate on experimental autoimmune uveoretinitis.

TNFalpha is required for late BRB breakdown in diabetic retinopathy, and its inhibition prevents leukostasis and protects vessels and neurons from apoptosis.

Association of birth parameters with OCT measured macular and retinal nerve fiber layer thickness.

In vivo confocal microscopic evidence of keratopathy in patients with pseudoexfoliation syndrome.

Generation of Cre transgenic mice with postnatal RPE-specific ocular expression.

Geranylgeranylation facilitates proteasomal degradation of rho G-proteins in human trabecular meshwork cells.

Eye-hand coordination skills in children with and without amblyopia.

The therapeutic impact of perceptual learning on juvenile amblyopia with or without previous patching treatment.

Longitudinal change detected by spectral domain optical coherence tomography in the optic nerve head and peripapillary retina in experimental glaucoma.

Long-term in vivo imaging and measurement of dendritic shrinkage of retinal ganglion cells.

Lack of TNF-alpha promotes caspase-3-independent apoptosis during murine cytomegalovirus retinitis.

Hemianopic and quadrantanopic field loss, eye and head movements, and driving.

Sclera as a surrogate marker for determining AGE-modifications in Bruch's membrane using a Raman spectroscopy-based index of aging.

Genetic variants associated with optic nerve vertical cup-to-disc ratio are risk factors for primary open angle glaucoma in a US Caucasian population.

Choriocapillaris vascular dropout related to density of drusen in human eyes with early age-related macular degeneration.

Assumption constraints of fluorophotometry in human eyes.

Cell-deposited matrix improves retinal pigment epithelium survival on aged submacular human Bruch's membrane.

Tumor necrosis factor-alpha mediates photoreceptor death in a rodent model of retinal detachment.

Dark matters in AMD genetics: epigenetics and stochasticity.

How should we measure the ciliary muscle?

Understanding autoimmune uveitis through animal models. The Friedenwald Lecture.

Author response: On the presence of (O-acyl)-omega-hydroxy fatty acids and their esters in human meibomian gland secretions.

Year: 2011 Volume: 52 Issue: 2

Year: 2011 Volume: 52 Issue: 1

Year: 2010 Volume: 51

Year: 2009 Volume: 50

Year: 2008 Volume: 49

Sclera as a surrogate marker for determining AGE-modifications in Bruch's membrane using a Raman spectroscopy-based index of aging.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Beattie, J. Renwick McGarvey, John J. Stitt, Alan W. Pawlak, Anna M.
Description	Country affiliation: United kingdom Author Affiliation: Beattie JR ( Centre for Vision and Vascular Science, School of Medicine and Dentistry, Queen's University Belfast, Belfast, United Kingdom.)
Abstract	PURPOSE: Raman spectroscopy is an effective probe of advanced glycation end products (AGEs) in Bruch's membrane. However, because it is the outermost layer of the retina, this extracellular matrix is difficult to analyze in vivo with current technology. The sclera shares many compositional characteristics with Bruch's membrane, but it is much easier to access for in vivo Raman analysis. This study investigated whether sclera could act as a surrogate tissue for Raman-based investigation of pathogenic AGEs in Bruch's membrane. METHODS: Human sclera and Bruch's membrane were dissected from postmortem eyes (n = 67) across a wide age range (33-92 years) and were probed by Raman spectroscopy. The biochemical composition, AGEs, and their age-related trends were determined from data reduction of the Raman spectra and compared for the two tissues. RESULTS: Raman microscopy demonstrated that Bruch's membrane and sclera are composed of a similar range of biomolecules but with distinct relative quantities, such as in the heme/collagen and the elastin/collagen ratios. Both tissues accumulated AGEs, and these correlated with chronological age (R(2) = 0.824 and R(2) = 0.717 for sclera and Bruch's membrane, respectively). The sclera accumulated AGE adducts at a lower rate than Bruch's membrane, and the models of overall age-related changes exhibited a lower rate (one-fourth that of Bruch's membrane) but a significant increase with age (P < 0.05). CONCLUSIONS: The results suggest that the sclera is a viable surrogate marker for estimating AGE accumulation in Bruch's membrane and for reliably predicting chronological age. These findings also suggest that sclera could be a useful target tissue for future patient-based, Raman spectroscopy studies.
ISSN	01460404
e-ISSN	15525783
DOI	10.1167/iovs.10-6554
Journal	Investigative Opthalmology & Visual Science
Issue Number	3
Volume Number	52
Language	English
Publisher	Association for Research in Vision and Ophthalmology
Publisher Date	2011-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Aging Physiology Biological Markers Metabolism Bruch Membrane Glycosylation End Products, Advanced Sclera Eye Proteins Spectrum Analysis, Raman Tissue Donors Beta Carotene Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Ophthalmology
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in