NDLI: Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium

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Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium

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Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rao, Veena S. Rizzolo, Lawrence J. Adelman, Ron A. Peng, Shaomin
Description	Country affiliation: United States Author Affiliation: Peng S ( Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06520-8062, USA.)
Abstract	PURPOSE: The retinal pigment epithelium (RPE) separates photoreceptors from choroidal capillaries, but in age-related macular degeneration (AMD) capillaries breach the RPE barrier. Little is known about human RPE tight junctions or the effects of serum on the retinal side of the RPE. METHODS: Cultured human fetal RPE (hfRPE) was assessed by the transepithelial electrical resistance (TER) and the transepithelial diffusion of methylated polyethylene glycol (mPEG). Claudins and occludin were monitored by quantitative RT-PCR, immunoblotting, and immunofluorescence. RESULTS: Similar to freshly isolated hfRPE, claudin-19 mRNA was 25 times more abundant than claudin-3. Other detectable claudin mRNAs were found in even lesser amounts, as little as 3000 times less abundant than claudin-19. Claudin-1 and claudin-10b were detected only in subpopulations of cells, whereas others were undetectable. Knockdown of claudin-19 by small interfering RNA (siRNA) eliminated the TER. siRNAs for other claudins had minimal effects. Serum affected tight junctions only when presented to the retinal side of the RPE. The TER increased 2 times, and the conductance of K(+) relative to Na(+) decreased without affecting the permeability of mPEG. These effects correlated with increased steady-state levels of occludin. CONCLUSIONS: Fetal human RPE is a claudin-19-dominant epithelium that has regional variations in claudin-expression. Apical serum decreases RPE permeability, which might be a defense mechanism that would retard the spread of edema due to AMD.
ISSN	01460404
e-ISSN	15525783
DOI	10.1167/iovs.10-5984
Journal	Investigative Opthalmology & Visual Science
Issue Number	3
Volume Number	52
Language	English
Publisher	Association for Research in Vision and Ophthalmology
Publisher Date	2011-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Membrane Proteins Physiology Retinal Pigment Epithelium Metabolism Tight Junctions Cells, Cultured Claudins Electric Impedance Fluorescent Antibody Technique, Indirect Gene Silencing Gestational Age Immunoblotting Genetics Occludin Permeability Polyethylene Glycols Rna, Messenger Rna, Small Interfering Pharmacology Embryology Reverse Transcriptase Polymerase Chain Reaction Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Ophthalmology
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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