NDLI: Engineering cell motility via substrate incorporation of poly(ethylene glycol)

Content Provider	IEEE Xplore Digital Library
Author	Sharma, R.I. Kohn, J. Moghe, P.V.
Copyright Year	2002
Description	Author affiliation: Dept. of Biomed. Eng., Rutgers Univ., Piscataway, NJ, USA (Sharma, R.I.; Kohn, J.; Moghe, P.V.)
Abstract	This study examined the modulation of cell migratory responsiveness to protein-adsorbed poly(ethylene glycol) (PEG) variant copolymers. To this end, fibronectin was adsorbed to equivalent levels on copolymers based on a family of tyrosine/PEG derived polycarbonates. Atomic force microscopy (AFM) revealed unique protein distributions for each copolymer system, indicating that although equal amounts of ligand are presented, the concentration of PEG in the underlying substrate can effect the adsorbed ligand. Consequently, quantitation of fibronectin RGD-binding sites on the protein-adsorbed copolymers revealed that cell-binding sites were maximized at intermediate concentrations of PEG. Our migration studies demonstrated that increasing PEG in the copolymer increased cell speed monotonically up to an intermediate PEG level where a maximum speed was reached. After incubating cells on ligand adsorbed copolymers, higher PEG levels elicited equivalent number of cell-binding sites relative to those on intermediate levels of PEG. Integrin inhibition studies showed that /spl alpha//sub 5//spl beta//sub 1/ was critical in modulating the number of RGD sites. Our AFM data suggests that rearrangement at higher PEG levels was facilitated by electrostatic repulsion, effecting fibronectin slippage at the polymer surface as well as conformational rearrangement revealing addition al RGD binding sites. Overall, we report on the finding that cell migratory responsiveness to a given ligand concentration can be further manipulated via systematic ligand changes due to the substrate PEG composition.
Starting Page	765
Ending Page	766
File Size	178161
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1137060
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Atomic force microscopy Substrates Delay Instruments Surface topography Protein engineering Electrostatics Polymers Wounds Adhesives
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Poly(ethylene glycol) interfaces for the control of biofouling in silicon-based microsystems

Poly(ethylene glycol) enhances cell motility on protein-based poly(ethylene glycol)-polycarbonate substrates: a mechanism for cell-guided ligand remodeling.

The influence of surface morphology and rigidity of the substrata on cell motility

Nanomanipulation using atomic force microscope with drift compensation

Investigating the adsorption mechanism of Bovine Serum Albumin on crystal surface by steering atomic force microscopy

Limits of high-density, low-force pressure contacts

Study of electrostatic properties of dielectric surfaces and powders using scanning probe microscopy

Poly(ethylene oxide) surfactant polymers

Characterization of etched and Unetched Vertically Aligned Carbon Nanofibers (VACNFs) using Atomic Force Microscopy

Engineering cell motility via substrate incorporation of poly(ethylene glycol)

Similar Documents

Poly(ethylene glycol) interfaces for the control of biofouling in silicon-based microsystems

Poly(ethylene glycol) enhances cell motility on protein-based poly(ethylene glycol)-polycarbonate substrates: a mechanism for cell-guided ligand remodeling.

The influence of surface morphology and rigidity of the substrata on cell motility

Nanomanipulation using atomic force microscope with drift compensation

Investigating the adsorption mechanism of Bovine Serum Albumin on crystal surface by steering atomic force microscopy

Limits of high-density, low-force pressure contacts

Study of electrostatic properties of dielectric surfaces and powders using scanning probe microscopy

Poly(ethylene oxide) surfactant polymers

Characterization of etched and Unetched Vertically Aligned Carbon Nanofibers (VACNFs) using Atomic Force Microscopy

Engineering cell motility via substrate incorporation of poly(ethylene glycol)