NDLI: Assessment of monocyte derived dendritic cell phenotype upon biomaterial contact in vitro

Content Provider	IEEE Xplore Digital Library
Author	Yoshida, M. Babensee, J.E.
Copyright Year	2002
Description	Author affiliation: Wallace H. Coulter Dept. of Biomed. Eng., Georgia Inst. of Technol., Atlanta, GA, USA (Yoshida, M.; Babensee, J.E.)
Abstract	Immune response remains a concern associated with the successful integration of tissue engineered device into living systems. Upon implantation, both living and non-living device components elicit a specific immune and a nonspecific inflammatory response, respectively. We hypothesize that biomaterial contact is a maturation stimulus for dendritic cells (DC), the antigen presenting cells that activate T cells and initiate adaptive immunity. Maturation of DC was reconstituted by culturing human blood monocytes with GM-CSF and IL-4 to induce differentiation into immature DC (iDC). iDC were then treated with 4 /spl mu/m poly(lactic-co-glycolic) acid microparticles (PLGA MP), or with LPS to yield mature DC (mDC) as positive control. Cell morphology and surface phenotype were monitored as a function of time. On day 10, biomaterial- treated cells morphologically most closely resembled mDC with dendritic processes. In contrast and as expected, monocytes/macrophages did not exhibit processes and expressed high levels of CD14. mDC expressed high levels of costimulatory and MHC molecules compared to iDC. Our preliminary results show that biomaterial microparticles were phagocytosed, and the level of costimulatory and MHC molecules expression by biomaterial- treated cells was between that expressed by iDC and mDC.
Starting Page	619
Ending Page	620
File Size	188246
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1136982
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	In vitro Immune system Blood Surface morphology Biomedical engineering Pathogens Cells (biology) Humans Surface treatment Monitoring
Content Type	Text
Resource Type	Article

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