NDLI: Kidney cell mechanotransduction in response to sustained hydrostatic pressure

Content Provider	IEEE Xplore Digital Library
Author	Martin, J.S. Yokota, H. Haberstroh, K.M.
Copyright Year	2002
Description	Author affiliation: Dept. of Biomed. Eng., Purdue Univ., West Lafayette, IN, USA (Martin, J.S.)
Abstract	Several kidney pathologies result in elevated pressures within the renal interstitial fluid, affecting cell function and gene expression. The long-term objective of this research was, therefore, to elucidate the signaling cascades involved in kidney cell responses to hydrostatic (physiological and/or pathological) pressures. Initial in vitro studies were performed on two kidney cell lines (tubular and medullary) to determine changes in cell proliferation, cytoskeletal (specifically, microfilament and microtubule) arrangement, and mRNA expression (of cell signaling genes such as G/sub /spl alpha//, G/sub /spl beta//, c-jun, and c-fos) following exposure to pathological (60 cmH/sub 2/O) pressures. Proliferation studies provided evidence that exposure to pressures of 60 cmH/sub 2/O resulted in increased renal cell proliferation (compared to controls cells under 0.3 cmH/sub 2/O) after 7 days. In addition to increased cell proliferation, time-dependent changes in individual microfilament structures took place in response to the pressure stimulus. These results prove that hydrostatic pressure alters the function of tubular and medullary cells; more importantly, microfilaments were involved in the signaling mechanism of this response. Additionally, preliminary results suggest that G/sub /spl alpha//, G/sub /spl beta//, c-jun, and c-fos are up-regulated in renal cells following exposure to 60 cmH/sub 2/O pressure. Uncovering this signaling cascade in renal cells may aid in the design of novel, targeted drug therapies for kidney pathologies.
Starting Page	327
Ending Page	328
File Size	167451
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1134518
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Pathology Cells (biology) Pressure control Biomedical engineering Signal design Drugs Medical treatment Blood pressure Biomedical monitoring Gene expression
Content Type	Text
Resource Type	Article

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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