NDLI: Effects of electric pulse strength and pulse duration on plasmid DNA electromobility

Content Provider	IEEE Xplore Digital Library
Author	Zaharoff, D.A. Fan Yuan
Copyright Year	2002
Description	Author affiliation: Dept. of Biomed. Eng., Duke Univ., Durham, NC, USA (Zaharoff, D.A.; Fan Yuan)
Abstract	Interstitial transport of DNA is a rate-limiting step in electric field-mediated gene delivery. Interstitial transport of macromolecules such as plasmid DNA is limited by small diffusion coefficient, large diffusion distance and inadequate convection. Here, we explore electric field as a novel interstitial driving force for plasmid DNA transport. We measured the electrophoretic movement, and hence, electromobility of fluorescently-labeled plasmid DNA in agarose gels of three concentrations (1, 2, and 3%), subjected to electric pulses at three levels of field strength (100, 200 and 400 V/cm) and four levels of pulse duration (10, 50, 75, 99 ms). In our experiments, electrophoresis is up to four orders of magnitude greater than diffusion for plasmid transport in agarose gel. In all gels, we found that shorter duration pulses (<10 ms) are not as efficient as longer duration pulses for pushing DNA. We also found that electromobility increases monotonically with applied voltage resulting in a maximum value at the highest applied voltage (400 V/cm) in all three gels. The rate of electromobility increase with applied voltage was amplified at higher gel concentration. These results indicate that plasmid DNA electromobility in porous media can be optimized through pulse duration and pulse strength.
Starting Page	546
Ending Page	547
File Size	109169
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1136942
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	DNA Pulse measurements Pulse amplifiers Voltage Electrokinetics Fluorescence In vivo Electric variables measurement Motion measurement Neoplasms
Content Type	Text
Resource Type	Article

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