NDLI: Induced cell clustering enhances islet /spl beta/ cell formation from pancreatic ductal epithelial cells

Content Provider	IEEE Xplore Digital Library
Author	Boretti, M. Gooch, K.J.
Copyright Year	2002
Description	Author affiliation: Dept. of Bioeng., Pennsylvania Univ., Philadelphia, PA, USA (Boretti, M.; Gooch, K.J.)
Abstract	Although islet transplantation holds great promise as a treatment for diabetes, a major limitation to its future clinical use is a shortage of donor tissue. During both fetal development and adult pancreatic regeneration, pancreatic ductal epithelial cells (PDEC) differentiate into endocrine cells. The goal of this project is to apply existing understanding of these processes to stimulate islet cell differentiation in vitro. Following 11-14 days of culturing adult human PDEC in a standard cell culture environment (tissue culture treated plastic, 10% FBS), they transdifferentiate to /spl beta/ cells at an extremely low efficiency (0.1%). /spl beta/-cell differentiation was enhanced greater than 4-fold (0.42%) when PDEC were grown on a low adhesive substrate (nontreated plastic) and in a low serum medium (5% Nu serum), conditions which are permissive to cell detachment and clustering. While the overall efficiency of transdifferentiation was still very low, the results suggest that induced cell-cell interactions and cell shape changes may be critical to stimulating /spl beta/-cell production in vitro. We are currently exploring additional factors (ECM factors, growth factors, transcription factors) that may improve the efficiency of transdifferentiation in order to optimize the creation of tissue-engineered islets.
Starting Page	892
Ending Page	893
File Size	233061
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1137130
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Pancreas Insulin Diabetes Plastics Sugar Humans Medical treatment In vitro Cardiovascular diseases Biomedical engineering
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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