NDLI: Dynamic behavior of nerve growth factor upon administration to the rat brain parenchyma

Content Provider	IEEE Xplore Digital Library
Author	Stroh, M.A. Saltzman, W.M.
Copyright Year	2002
Description	Author affiliation: Dept. of Chem. Eng., Cornell Univ., Ithaca, NY, USA (Stroh, M.A.; Saltzman, W.M.)
Abstract	By understanding how neurotrophins navigate through brain tissue, we move closer to unlocking the potential therapeutic benefit of these small (26-28kD), basic proteins. We utilize two-photon laser scanning microscopy (TPLSM) to characterize the temporal and spatial fate of the prototypical neurotrophin, nerve growth factor, in brain tissue. We administer a rhodamine-nerve growth factor conjugate (RNGF) from a micropipette to the striatal region of rat brain tissue slices. Two-photon imaging allows us to image deep into 400 micron-thick tissue slices with minimal photodamage of tissue and photobleaching of label. We use a point-source diffusion model to describe the distribution of RNGF and estimate an apparent brain tissue diffusion coefficient (D/sub B/(34/spl deg/C)) of 2.75/spl times/10/sup -7/cm/sup 2//sec. We utilize two-photon fluorescence photobleaching recovery (FPR) to estimate the free diffusion coefficient in water (D/sub F/(34/spl deg/C)) of 8.79/spl times/10/sup -7/cm/sup 2//sec. The extracellular space creates a tortuous path for diffusion, and an anticipated mismatch between D/sub F/ and D/sub B/. The square-root of the ratio of D/sub F/ to D/sub B/ gives the tortuosity, /spl tau/. We compare our estimate of /spl tau/ (1.79) to literature values for other proteins, and conclude that NGF enjoys relatively rapid diffusion in tissue. We extrapolate these findings to postulate formulation strategies for eventual clinical application.
Starting Page	518
Ending Page	519
File Size	181407
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1136926
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Brain Photobleaching Fluorescence Navigation Microscopy Extracellular Chemical engineering Protein engineering Chemical lasers Prototypes
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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