NDLI: Flow-induced strain focusing in the endothelial cytoskeleton

Content Provider	IEEE Xplore Digital Library
Author	Helmke, B.P. Rosen, A.B. Choi, K. Davies, P.F.
Copyright Year	2002
Description	Author affiliation: Dept. of Biomed. Eng., Virginia Univ., Charlottesville, VA, USA (Helmke, B.P.)
Abstract	Displacement or deformation of the endothelial cell cytoskeleton by hemodynamic shear stress may reflect redistribution of intracellular force to molecular signaling complexes that initiate adaptive mechanisms. In these studies, cytoskeletal movement in response to onset of shear stress was measured in endothelial cells expressing GFP-vimentin or GFP-actin. High-resolution optical sectioning fluorescence microscopy and quantitative image processing revealed increased intermediate filament displacement with height in the cell and in downstream regions of the cytoplasm. The Lagrangian strain tensor demonstrated highly localized deformation near the coverslip. Variability in principal strain orientation increased significantly after onset of shear stress. In contrast to the spatially heterogeneous distribution of displacement and strain in the intermediate filament network, onset of shear stress induced rapid extension of actin ruffles in the upstream direction, and lamellipodia along downstream edges of the cell were often retracted. This directional response often subsided within 10-15 min and occurred in cells in confluent but not subconfluent monolayers. In addition, microfilaments in central regions of the cytoplasm were often displaced by flow onset. These data suggest that endothelial cells sense changes in shear stress and rapidly mobilize an active cytoskeletal response that may contribute to initiation of adaptive mechanotransduction networks.
Starting Page	321
Ending Page	322
File Size	172973
Page Count	2
File Format	PDF
ISBN	0780376129
ISSN	1094687X
DOI	10.1109/IEMBS.2002.1134514
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-23
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Capacitive sensors Optical microscopy Focusing Hemodynamics Stress measurement Optical sensors Fluorescence Image processing Lagrangian functions Tensile stress
Content Type	Text
Resource Type	Article

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