NDLI: Independent domain folding of Pseudomonas exotoxin and single-chain immunotoxins: influence of interdomain connections.

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Independent domain folding of Pseudomonas exotoxin and single-chain immunotoxins: influence of interdomain connections.

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Independent domain folding of Pseudomonas exotoxin and single-chain immunotoxins: influence of interdomain connections.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pastan, I. Brinkmann, U. Buchner, J.
Description	Author Affiliation: Brinkmann U ( Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.);
Abstract	We have studied the refolding of completely unfolded and reduced Pseudomonas exotoxin (PE) and of recombinant single-chain immunotoxins made with monoclonal antibody B3 that are composed of a heavy-chain variable region connected by a flexible linker to the corresponding light-chain variable region (Fv), which is in turn fused to a truncated form of PE. We have found by direct activity assays that different functional domains of these multifunctional proteins fold independently with different kinetics. The ADP-ribosylation domain of PE and of the recombinant immunotoxin fold rapidly, whereas the assembly of the binding and/or translocation domains is regained more slowly. The complete refolding of native PE occurs more rapidly than the refolding of the recombinant immunotoxins. To determine the influence of the connector region between the B3(Fv) moiety and the toxin on the folding process of the recombinant immunotoxin B3(Fv)-PE38KDEL, we have made two different mutations in the peptide that connects the single-chain Fv domain to domain II of PE. These molecules show different folding kinetics, differences in their propensity to aggregate, and different yields of correctly folded molecules. A mutation that decreases aggregation increases the rate of formation and the yield of active immunotoxin molecules.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	7
Volume Number	89
Language	English
Publisher	National Academy of Sciences
Publisher Date	1992-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	ADP Ribose Transferases Exotoxins Chemistry Immunotoxins Virulence Factors Amino Acid Sequence Bacterial Toxins Macromolecular Substances Molecular Sequence Data Mutagenesis, Site-Directed Oligodeoxyribonucleotides Poly(ADP-ribose) Polymerases Metabolism Protein Binding Protein Denaturation Pseudomonas Aeruginosa Recombinant Fusion Proteins Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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