NDLI: Infectious Sindbis virus transient expression vectors for studying antigen processing and presentation.

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Infectious Sindbis virus transient expression vectors for studying antigen processing and presentation.

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Infectious Sindbis virus transient expression vectors for studying antigen processing and presentation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rice, C. M. Hahn, C. S. Braciale, T. J. Hahn, Y. S.
Description	Author Affiliation: Hahn CS ( Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110-1093.);
Abstract	Sindbis virus (SIN) is a small positive-strand enveloped RNA virus that infects a broad range of vertebrate and insect cells. A SIN vector (called dsSIN), designed for transient expression of heterologous RNAs and proteins, was engineered by inserting a second subgenomic mRNA promoter sequence into a nonessential region of the SIN genome. By using this vector, dsSIN recombinants have been constructed that express either bacterial chloramphenicol acetyltransferase, a truncated form of the influenza hemagglutinin (HA), or mini-genes encoding two distinct immunodominant cytotoxic T lymphocyte (CTL) HA epitopes. Infection of murine cell lines with these recombinants resulted in the expression of approximately 10(6)-10(7) chloramphenicol acetyltransferase polypeptides per cell and efficient sensitization of target cells for lysis by appropriate major histocompatibility complex-restricted HA-specific CTL clones in vitro. In addition, priming of an influenza-specific T-cell response was observed after immunizing mice with dsSIN recombinants expressing either a truncated form of HA or the immunodominant influenza CTL epitopes. This SIN expression system allows the generation of high-titered recombinant virus stocks in a matter of days and should facilitate mapping and mutational analysis of class I major histocompatibility complex-restricted T-cell epitopes expressed via the endogenous pathway of antigen processing and presentation.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	7
Volume Number	89
Language	English
Publisher	National Academy of Sciences
Publisher Date	1992-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antigen-Presenting Cells Immunology Antigens Metabolism Genetic Vectors Sindbis Virus Genetics Amino Acid Sequence Animals Cytotoxicity, Immunologic DNA, Recombinant Epitopes H-2 Antigens Hemagglutinins, Viral In Vitro Techniques Influenza A Virus Mice Molecular Sequence Data Growth & Development T-Lymphocytes, Cytotoxic Virus Replication Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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