NDLI: Activation of mechanosensitive transcription factors in murine C2C12 myoblasts by focused low-intensity pulsed Ultrasound (FLIPUS)

Content Provider	IEEE Xplore Digital Library
Author	Puts, R. Rikeit, P. Ruschke, K. Kadow-Romacker, A. Soyoung Hwang Jenderka, K.-V. Knaus, P. Raum, K.
Copyright Year	2015
Description	Author affiliation: Berlin-Brandenburg Sch. for Regenerative Therapies, Charite - Universitatsmed. Berlin, Berlin, Germany (Puts, R.; Rikeit, P.; Kadow-Romacker, A.; Knaus, P.; Raum, K.) \|\| Dept. of Diagnostics & Ultrasound, Merseburg Univ. of Appl. Sci., Merseburg, Germany (Jenderka, K.-V.) \|\| Dept. of Biotechnol., Tech. Univ. Berlin, Berlin, Germany (Soyoung Hwang) \|\| Inst. for Chem. & Biochem., Freie Univ. Berlin, Berlin, Germany (Ruschke, K.)
Abstract	Quantitative Ultrasound (QUS) is a promising technique for bone tissue evaluation. Focused transducers used for QUS also have a capability to be applied for tissue-regenerative purposes and can provide spatially limited deposition of acoustic energy. We describe a focused LIPUS (FLIPUS) system, which has been developed for the stimulation of cell monolayers in the defocused far field of the transducer through the bottom of the well plate. Tissue culture well plates, carrying the cells, were incubated in a special chamber, immersed in a temperature controlled water tank. The ultrasound was introduced at 3.6 MHz, pulsed at 100 Hz repetition frequency with 27.8% duty cycle. The calibrated output intensity corresponded to ISATA = 44.5 ± 7.1 $mW/cm^{2}.$ In this study we evaluated mechanical response of murine C2C12 myoblastic cells to ultrasound. FLIPUS induced 1.5 fold increase (p<;0.05) in AP-1 promoter activity already after 5-minute of FLIPUS exposure. The Sp1 binding was enhanced by about 20% (p<;0.05) after 5-min FLIPUS stimulation and the trend persisted for 30 min (p<;0.01) and 1 hour (p<;0.01) stimulation times. Increase of 70% in activation of TEAD-binding sequences (p<;0.01) was observed after the 5 min FLIPUS treatment. The proliferation of the C2C12 cells was up-regulated after the 5-minlong FLIPUS exposure. These results imply the pro-proliferative nature of the selected FLIPUS-intensity in C2C12s, with potential functional role of the described mechanosensitive transcription factors. The use of FLIPUS as a visualization and regenerative technique can be further applied in clinical settings as a device providing monitoring and healing of the damaged tissue.
Starting Page	1
Ending Page	4
File Size	467675
Page Count	4
File Format	PDF
e-ISBN	9781479981823
DOI	10.1109/ULTSYM.2015.0413
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2015-10-21
Publisher Place	Taiwan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Acoustics Transducers Proteins Ultrasonic imaging Acoustic measurements Temperature measurement Biomembranes regeneration LIPUS QUS mechanosensation bone muscle
Content Type	Text
Resource Type	Article

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