NDLI: An ImageJ plugin for the sizing and counting of microbubbles

Content Provider	IEEE Xplore Digital Library
Author	Sennoga, C.A. Kanbar, E. Bouakaz, A.
Copyright Year	2015
Description	Author affiliation: Inserm, Univ. Francois-Rabelais de Tours, Tours, France (Sennoga, C.A.; Kanbar, E.; Bouakaz, A.)
Abstract	Accumulating evidence demonstrates a strong correlation between ultrasound contrast image enhancement and the size distribution of the microbubble ultrasound contrast agent used. Accurate determination of the size and concentration of microbubble ultrasound contrast agents is therefore important, not only for the experimental verification of theories on nonlinear bubble oscillations but also for the routine assessment of their quality control. In the present work, we have assembled an open-source ImageJ plugin designed to identify and determine the diameter, size distribution and concentration of microbubbles using bright field microscopy images of dispersed microbubbles. The plug-in named “BubblesizerJ” uses image segmentation combined with existing algorithms for particle size determination and spheroid distance transform to identify, count and size microbubbles. BubblesizerJ was validated using digital synthetic images of black circles on a white background and bright field microscopy images of nominally monodispersed microspheres of known modal diameter and concentration. Demonstrating the utility of the plugin, was achieved by repeatedly determining the size and concentration of $SonoVue^{®},$ a commercial ultrasound contrast agent. We have applied the plugin to the analyses of microscopic images with differing resolution and quality and it consistently returned diameters and concentrations not statistically different from known values. We demonstrated that the plugin enables users to rapidly and thoroughly determine the concentration and diameter of microbubbles.
Starting Page	1
Ending Page	4
File Size	1249958
Page Count	4
File Format	PDF
e-ISBN	9781479981823
DOI	10.1109/ULTSYM.2015.0483
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2015-10-21
Publisher Place	Taiwan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Image resolution Ultrasonic imaging Microscopy Algorithm design and analysis Calibration Transforms Software BubblesizerJ microbubbles size distribution concentration imageJ microscopy
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

An improved methodology for blood cell counting

An interactive ImageJ plugin for semi-automated image denoising in electron microscopy

A Wavelet-Based EM Algorithm for the Restoration of Medical Pulse-Echo Ultrasound Datasets

SOFTWARE Open Access IPLaminator: an ImageJ plugin for

Reconfigurable architecture for ultrasonic signal compression and target detection

TACI: an ImageJ plugin for 3D calcium imaging analysis

Designing and Programming an Efficient Software for Sizing and Counting Various Particles using Image Processing Technique

Real-time optical imaging of individual microbubbles in an ultrasonic field

3D-4 Analysis of Ultrasonic 3-D Image Compression Using Non-Uniform, Separable Wavelet Transforms

An ImageJ plugin for the sizing and counting of microbubbles

Similar Documents

An improved methodology for blood cell counting

An interactive ImageJ plugin for semi-automated image denoising in electron microscopy

A Wavelet-Based EM Algorithm for the Restoration of Medical Pulse-Echo Ultrasound Datasets

SOFTWARE Open Access IPLaminator: an ImageJ plugin for

Reconfigurable architecture for ultrasonic signal compression and target detection

TACI: an ImageJ plugin for 3D calcium imaging analysis

Designing and Programming an Efficient Software for Sizing and Counting Various Particles using Image Processing Technique

Real-time optical imaging of individual microbubbles in an ultrasonic field

3D-4 Analysis of Ultrasonic 3-D Image Compression Using Non-Uniform, Separable Wavelet Transforms

An ImageJ plugin for the sizing and counting of microbubbles