NDLI: A micro-fabricated capillary electrophoresis chip for DNA pre-concentration and separation

Content Provider	IEEE Xplore Digital Library
Author	Chen-Hua Kuo Jung-Hao Wang Gwo-Bin Lee
Copyright Year	2009
Description	Author affiliation: Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan 701 (Chen-Hua Kuo; Jung-Hao Wang; Gwo-Bin Lee)
Abstract	A simple online DNA pre-concentration and separation method by using a micro capillary electrophoresis (CE) chip integrated with a normally-closed valve activated by pneumatic suction has been developed. The normally-closed valve with anionic surface charges forms a nanoscale channel only allows the passage of electric current but traps the DNA samples so that they can be pre-concentrated. Obvious fluorescent-labeled DNA concentration is observed at the pre-concentration area. The fX174 DNA/HaeIII markers separation signals are significantly enhanced comparing with the results of a traditional cross-shaped micro CE chip. Therefore, successful DNA pre-concentration for signal enhancement and separation analysis can be performed by using this new micro CE chip.
Starting Page	366
Ending Page	369
File Size	651227
Page Count	4
File Format	PDF
ISBN	9781424441907
e-ISBN	9781424441938
DOI	10.1109/SENSOR.2009.5285486
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-06-21
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrokinetics DNA Valves Surface treatment Strontium Reservoirs Electric potential Current Chip scale packaging Voltage pre-concentration Micro capillary electrophoresis DNA separation normally-closed valve
Content Type	Text
Resource Type	Article

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