NDLI: PPPS-2013: Nanoelectroablation for human carcinoma therapy

Content Provider	IEEE Xplore Digital Library
Author	Nuccitelli, R. Kreis, M. Athos, B. Lui, K. Berridge, C. Nuccitelli, P. Epstein, E.
Copyright Year	2013
Description	Author affiliation: Children's Hosp. Oakland Res. Inst., Oakland, CA, USA (Epstein, E.) \|\| BioElectroMed Corp., Burlingame, CA, USA (Nuccitelli, R.; Kreis, M.; Athos, B.; Lui, K.; Berridge, C.; Nuccitelli, P.)
Abstract	We have developed a low energy direct current pulsed electric field therapy for tissue ablation. This therapy applies 100 ns long electric pulses 30 kV/cm in amplitude using a contact electrode and triggers apoptosis in the treated tissue. Here we review the progress that has been made in understanding the mechanisms and targets of nanosecond pulsed electric fields (nsPEF) when applied to cells and tissues. This work began in 2001 in the laboratory of Karl Schoenbach who collaborated with biologists Stephen Beebe and Stephen Buescher to demonstrate the permeabilization of intracellular organelles. Since then over 100 papers have been published studying the cellular responses to nsPEF. We discuss these targets and cellular responses and introduce some new results from our group using nanoelectroablation to treat human pancreatic carcinoma in a murine xenograft model system. We have determined that 500 pulses 100 ns long and 30 kV/cm in amplitude are sufficient to ablate human pancreatic carcinomas growing in immunosuppressed mice and these ablated tumors do not recur for at least 300 days. We have also determined that the reactive oxygen species generation that is triggered within a minute after nsPEF treatment is $Ca^{2+}-dependent.$ In order bring this therapy into the clinic for the treatment of human tumors we are developing both a pulse generator as well as delivery electrodes to target the tumors to be treated. We will describe the $NanoBlate^{®}$ Model NB-1 100 ns pulse generator and the first human clinical trial data using nanoelectroablation to ablate basal cell carcinomas without scarring.
Starting Page	1
Ending Page	1
File Size	98697
Page Count	1
File Format	PDF
ISBN	9781467351713
ISSN	07309244
DOI	10.1109/PLASMA.2013.6635204
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-06-16
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Nanobioscience Tumors Electric fields Electrodes Generators Hospitals
Content Type	Text
Resource Type	Article
Subject	Atomic and Molecular Physics, and Optics Condensed Matter Physics Electrical and Electronic Engineering

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