NDLI: Non-thermal plasma treatment changes proteome signature and triggers cell signaling in human cells

Content Provider	IEEE Xplore Digital Library
Author	Wende, K. Bundscherer, L. Ottmuller, K. Schmidt, A. Weltmann, K.-D. Masur, K. Hochgrafe, F.
Copyright Year	2013
Description	Author affiliation: Center for Innovation Competence plasmatis, INP Greifswald e.V., Greifswald, Germany (Wende, K.; Bundscherer, L.; Ottmuller, K.; Schmidt, A.; Weltmann, K.-D.; Masur, K.) \|\| Inst. of Microbiol., Ernst Moritz Arndt Univ. of Greifswald, Greifswald, Germany (Hochgrafe, F.)
Abstract	Non thermal atmospheric pressure plasmas produce reactive oxygen and nitrogen species (ROS/RNS) which can influence cell physiology and trigger cellular signaling processes. This opens exiting new potentials e.g. in chronic wound care where encouraging results have been obtained in vivo by different research groups. Other data suggest the usability of plasma for anti cancer treatment. The fate of a eukaryotic cell after a challenge is determined by numerous factors. Important roles play the mitogen-activated protein kinases (MAPK). This enzyme group orchestrates cellular behavior upon activation by external stimuli (growth factors, chemicals, radiation), leading either to apoptosis or cell cycle arrest or to survival and differentiation. To gain insight into the processes triggered by non thermal plasma treatment (argon driven kinpen), human keratinocytes and lymphocytes were investigated using different proteomic tools. Protein expression pattern were determined using SILAC and label free high resolution mass spectrometry coupled to liquid chromatography. MAPK signaling pathways were investigated by western blotting technique. An ambivalent behavior of the cells was observed. Protein expression pattern was found to change distinctly 6 h to 8 h after treatment. Differences were less obvious after 3 h and 24 h of incubation. Major changes were identified for proteins involved in metabolism, protein expression, and oxidative stress response. ROS/RNS signaling was observed, together with downstream changes in ROS scavenging enzymes. The activation of different MAPK (p38 MAPK, ERK, JNK) was influenced in a time dependent manner after treatment. Pro survival signaling ERK activation was observed immediately after plasma treatment and decreased afterwards. This indicates a possible activation which was also seen in active protein expression pathways. On the other hand, pro-apoptotic JNK activation increased after 3 to 6 hours after treatment. Hence, depending on the treatment time NTP can be used as a stimulus in order to activate cell response either towards survival or cell death. In more complex systems like a tissue, the observed signaling events can activate adjacent cells or cell groups - e.g. to stimulate keratinocyte proliferation or immune response.
Starting Page	1
Ending Page	1
File Size	109352
Page Count	1
File Format	PDF
ISBN	9781467351713
ISSN	07309244
DOI	10.1109/PLASMA.2013.6633196
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-06-16
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Plasmas Indium phosphide Technological innovation Educational institutions Nitrogen
Content Type	Text
Resource Type	Article
Subject	Atomic and Molecular Physics, and Optics Condensed Matter Physics Electrical and Electronic Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in