NDLI: Prokaryotic expression of the antigenic site B/C of S gene of porcine transmissible gastroenteritis virus and preparation of its monoclonal antibodies

Content Provider	IEEE Xplore Digital Library
Author	Xiaohua Nie Huanrong Li Ran Gao Wang Xin Li Zhang
Copyright Year	2011
Description	Author affiliation: Lab of Veterinary microbiology, CAU-BUA TCVM, Teaching & Research Team, College of Animal Science and Technology, Beijing University of Agriculture, China, 102206 (Xiaohua Nie; Huanrong Li; Ran Gao; Wang Xin; Li Zhang)
Abstract	In this study, the antigenic site B/C of S gene of transmissible gastroenteritis virus (TGEV) was cloned into prokaryotic expression vector pET-28a and the recombinant plasmid was transferred into E.coli BL21 (DE3). The fusion protein was expressed after induction with IPTG. The SDS-PAGE purified protein was used as immunogen. Six-week-old BALB/c mice were immunized three times with recombinant protein. The hybridoma cell strain, designated 5G, steadily secreting monoclonal antibodies (McAbs) against the protein of site B/C, was obtained by hybridoma technique. It belonged to IgM subclass and could recognize the site B/C recombinant protein of S gene of TGEV. It was confirmed that the McAbs only could react with TGEV using indirect immunofluorescence assay (IFA) and indirect ELISA, indicating the McAbs had good specificity.
Starting Page	7010
Ending Page	7013
File Size	373185
Page Count	4
File Format	PDF
ISBN	9781424491728
e-ISBN	9781424491711
DOI	10.1109/RSETE.2011.5965978
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-06-24
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Prokaryotic expression RNA The antigenic site B/C DNA S gene Monoclonal antibodies Transmissible gastroenteritis virus Mice Immune system Strain
Content Type	Text
Resource Type	Article

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