NDLI: The effect of chemical activator on the cellulase hydrolysis in the high-solid substrate

Content Provider	IEEE Xplore Digital Library
Author	Hui Tian Jingting Tang Zhou Zheng Tian Yihong Da Chun Gong Zhijun Li Juan Yao
Copyright Year	2011
Description	Author affiliation: China Three Gorges University, Alan G.Macdiarmid, Institute of Renewable Energy, Yichang, 443002, China (Hui Tian; Jingting Tang; Zhou Zheng; Tian Yihong; Da Chun Gong) \|\| Angel Yeast Co. Ltd., Yichang, Hubei, 443002, China (Zhijun Li; Juan Yao)
Abstract	The effect of the different substrate concentration, enzymatic compositions, varied chemical activators on the cellulase hydrolysis process was studied. The results showed that (1) the effect of the enzymatic hydrolysis was vital when the substrate was more than 5% (2) the enzymatic composition had much more effect ont the yield of cellulase hydrolysis (3) $Mg^{2+},$ $Co^{2+}$ had obvious effect in the low concentration (<1mM/L) with increasing 30 g/L compared to blank sample(4) the surfactants PEG 2000 and Tween 80 also had notable effect on the enzymatic hydrolysis with increasing 23 g/L. The reduce sugar was obtained up to 115g/L with glucose concentration 88g/L when the enzyme was loaded according to U/g cellulose FPA 45: β-glucodase 25:xylase 800 with 20% substrate concentration, 120–150rpm, pH4.8, 50°C for 144h. The converting yield was up to 85%. The study on the the characterization of cellulase hydrolysis in the high-solid substrate had an important significance for the bioprocess of ligninocellulose.
Starting Page	7234
Ending Page	7237
File Size	352661
Page Count	4
File Format	PDF
ISBN	9781424491728
e-ISBN	9781424491711
DOI	10.1109/RSETE.2011.5966037
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-06-24
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Biological system modeling Cellulase Renewable energy resources High-solid substrate concentration Solids Biochemistry Chemical activators Substrates Chemicals Sugar
Content Type	Text
Resource Type	Article

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