NDLI: Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV) in transgenic potato and its immunogenicity in mice

Please wait, while we are loading the content...

Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV) in transgenic potato and its immunogenicity in mice

Content Provider	Semantic Scholar
Author	Ahn, Dong-Joo Youm, Jung Won Kim, Suk Weon Yoon, Won Kee Kim, Hyoung Chin Hur, Tai-Young Joung, Young Hee Jeon, Jae-Heung Kim, Hyun Soon
Copyright Year	2013
Abstract	Abstract : Transgenic plants have been tested as an alternative host for the production and delivery of experimentaloral vaccines. Here, we developed transgenic potatoes that express the major antigenic sites A and D of the glycoproteinS from transmissible gastroenteritis coronavirus (TGEV-S 0.7 ) under three expression vector systems. The DNAintegration and mRNA expression level of the TGEV-S 0.7 gene were confirmed in transgenic plants by PCR and northernblot analysis. Antigen protein expression in transgenic potato was determined by western blot analysis. Enzyme-linkedimmunosorbent assay results revealed that based on a dilution series of Escherichia coli-derived antigen, the transgenicline P-2 had TGEV-S 70. protein at levels that were 0.015% of total soluble proteins. We then examined theimmunogenicity of potato-derived TGEV-S 70. antigen in mice. Compared with the wild-type potato treated group andsynthetic antigen treated group, mice treated with the potato-derived antigen showed significantly higher levels ofimmunoglobulin (Ig) G and IgA responses.Keywords : oral administration, potato-derived antigen, transmissible gastroenteritis virus, transgenic plant
Starting Page	217
Ending Page	224
Page Count	8
File Format	PDF HTM / HTML
DOI	10.14405/kjvr.2013.53.4.217
Volume Number	53
Alternate Webpage(s)	http://ocean.kisti.re.kr/downfile/volume/ksvses/TSSOBU/2013/v53n4/TSSOBU_2013_v53n4_217.pdf
Alternate Webpage(s)	https://doi.org/10.14405/kjvr.2013.53.4.217
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in