NDLI: Determination of mineral elements in the embryos of normal and abnormal embryo of flare tree peony by ICP-AES

Content Provider	IEEE Xplore Digital Library
Author	Xiumei Zhou Baoyin Li
Copyright Year	2011
Description	Author affiliation: College of Horticulture and Landscape, Henan Institute of Science and Technology, Xinxiang, 453003, China (Xiumei Zhou; Baoyin Li)
Abstract	In order to find the cause of somatic embryo aberrance in the process of somatic embryogenesis in vitro of flare tree peony, the samples were digested by HNO3+HClO4(4∶1), 14 mineral elements (K, Na, Ca, Mg, Fe, Cu, Zn, Mn, Li, Ni, B, Ag, Cd, Pb) in normal embryos in vivo and the abnormal somatic embryos in vitro were determined by inductively coupled plasma-atomic emission spectrometry (ICP-AES). The results showed that the standard curves for 14 elements obtained were with good linearity, the measuring method was with high precision and multi-elements could be analyzed at the same time. 12 mineral elements (Ca, Mg, Cu, Zn, Mn, Fe, Pb, K, Na, B, Li, Ni) were determined in the normal embryo in vivo. While, among the 11 mineral elements (Ca, Mg, Zn, Mn, Fe, Pb, K, Na, B, Li, Cd) determined in the abnormal somatic embryo in vitro, there were 6 elements (Mg, Zn, Fe, K, Na, Li) that the contents were lower than those in the normal embryo, and the contents of Ca, Mn, Pb, B and Cd were higher than those in the normal one. Cu and Ni were not detected in the abnormal somatic embryo, but Cd was detected. These results can offer a reference on mineral elements for finding the causation of somatic embryo aberrance in the process of somatic embryogenesis in vitro of flare tree peony.
Starting Page	6728
Ending Page	6731
File Size	295872
Page Count	4
File Format	PDF
ISBN	9781424491728
e-ISBN	9781424491711
DOI	10.1109/RSETE.2011.5965905
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-06-24
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Abnormal somatic embryo in vitro Flare tree peony Embryo Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) Vegetation Lead Mineral Elements Iron Minerals Zinc Normal embryo in vivo Manganese
Content Type	Text
Resource Type	Article

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