NDLI: Cloning and function identification of the promoters and terminator of sugar beet chloroplast gene psbA

Content Provider	IEEE Xplore Digital Library
Author	Jie Cui Wenjing Li Dayou Cheng Xin Yang Binsheng Li
Copyright Year	2011
Description	Author affiliation: School of Food Science and Engineering of Harbin Institute Technology, China (Jie Cui; Wenjing Li; Dayou Cheng; Xin Yang) \|\| Forest botanical garden Heilongjiang, Harbin, China (Binsheng Li)
Abstract	Using two pairs of specific primers, DNA fragments containing the promoter PpsbA of psbA gene and terminator TpsbA of psbA gene, were obtained by PCR from Beta vulgaris. L chloroplast genomic DNA. Sequence analyzing results showed that the functional domains such as −35 domain, −10 domain and the translation control sequence motif in the cloned promoters the highly conserved to Nicotiana tabacum. The promoter PpsbA and the terminator TpsbA were cloned and the prokaryotic expression vectors pSKPTA of aadA gene were constructed by using PpsbA and the terminator TpsbA. Prokaryotic expression results showed that the promoters PpsbA cloned were functional and their activities could be controlled by the RNA polymerase of E.coli BL21 and the aadA controlled by them could be constitutively expressed E.coli in BL21.
Starting Page	6679
Ending Page	6681
File Size	408291
Page Count	3
File Format	PDF
ISBN	9781424491728
e-ISBN	9781424491711
DOI	10.1109/RSETE.2011.5965891
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-06-24
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Promoter Chloroplast gene psbA DNA Genomics Sugar beet Terminator Sugar industry Function Identification Cotton Bioinformatics
Content Type	Text
Resource Type	Article

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