NDLI: Ultra-high ratio dilution microfluidic system for single strand DNA isolation

Content Provider	IEEE Xplore Digital Library
Author	Christen, J.B. Iglehart, B. Pouliquen, P.
Copyright Year	2008
Description	Author affiliation: Immunogenetics Dept., Johns Hopkins Sch. of Med., Baltimore, MD (Christen, J.B.; Iglehart, B.)
Abstract	We present a system comprised of a PDMS microfluidic device and an injection system for dilution of DNA derived from whole blood to a single strand. We discuss two micro fluidic architectures for ultra-high ratio dilution (1:1000 or more) as well as the necessity to move beyond previously reported dilution architectures. We describe a custom injection system used both for fabrication and operation of the system. The system is operated by placing syringes, one filled with water and one filled with DNA extracted from whole blood, into the injection system. This system fixes and plunges the syringes simultaneously to introduce both solutions into the microfluidic channels that produce the ultra-high ratio dilutions. The most significant constraint on the design is that the system is designed for fabrication, characterization and use in a clinical setting.
Starting Page	1822
Ending Page	1825
File Size	413650
Page Count	4
File Format	PDF
ISBN	9781424416837
DOI	10.1109/ISCAS.2008.4541794
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-05-18
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microfluidics DNA Blood Fabrication Immune system Costs Humans Computer architecture Assembly systems Machining
Content Type	Text
Resource Type	Article

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