NDLI: Milligauss magnetic field applied pure water exert firefly luciferin-luciferase luminescence and induce intracellular calcium elevation of CHO cells without ATP

Content Provider	IEEE Xplore Digital Library
Author	Fukushima, M. Kataoka, T. Sugiyama, N. Mohri, K.
Copyright Year	1965
Abstract	In the previous paper, we demonstrated that milligauss ultra-low-frequency alternative current (mG ULF-AC) magnetic field applied phosphate buffered saline solution (PBS) per se stimulated elevation of intracellular Ca/sup 2+/ level and enhanced phagocytic activity of human poly-morphonuclear (PMN) leukocyte. During the course of the study to explore the mechanism of the biological effect of such mG magnetic field applied PBS, we found extraordinary phenomena which challenges our understanding of physical chemistry of water and bioenergetics. PBS and highly purified water were exposed to mG ULF-AC which was generated by a Helmholtz coil under visible light (mG/light). When mG/light-applied pure water or PBS was added to the medium, CHO cells which respond to exogenous adenosine triphosphate (ATP) resulting in increase of intracellular Ca/sup 2+/ level, were found to elevate intracellular Ca/sup 2+/ level without exogenous ATP. This activity of mG ULF-AC applied water or PBS was equivalent to 100 nM ATP. Furthermore, when magnetic field was applied on highly purified water under visible light, for more than 12 h at 40/spl deg/C, the pure water became to exert luminescence of luciferin in the presence of luciferase. This reaction did not require ATP nor Mg/sup 2+/ in the reaction mixture at all. These facts indicate that mG/light-applied PBS or water mimics ATP activity.
Sponsorship	IEEE Magnetics Society
Starting Page	4188
Ending Page	4190
Page Count	3
File Size	116413
File Format	PDF
ISSN	00189464
Volume Number	41
Issue Number	10
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2005-10-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Magnetic fields Luminescence Calcium Frequency Protons Photonics Cities and towns Humans Coils Cyclotrons water Bioluminescence energy conversion magnetic field effect
Content Type	Text
Resource Type	Article
Subject	Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering

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