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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Xu, J. J. Wang, P. Zhang, Y. J. Wang, J. L. |
| Spatial Coverage | China |
| Description | Country affiliation: China Author Affiliation: Zhang YJ ( Department of Gastroenterology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan Province, China zyjhkdyfy1268@163.com.); Xu JJ ( Department of Gastroenterology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan Province, China.); Wang P ( School of Public Health, Henan University of Technology, Luoyang, Henan Province, China.); Wang JL ( Department of Gastroenterology, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan Province, China.) |
| Abstract | We examined the relationship among the multidrug resistance (MDR1) gene product P-glycoprotein (P-gp), ulcerative colitis, and immune status under ulcerative colitis. MDR1 P-gp expression and interleukin-8 levels in ulcerative colitis were determined using immunohistochemistry and a double-antibody sandwich avidin-biotin complex-enzyme-linked immunosorbent assay, respectively. Nitric oxide content and nitric oxide synthase activity in the colonic mucosa were determined using a colorimetric method; CD4(+) and CD25(+) T cell subset percentages in the peripheral blood were determined by flow cytometry. The positive expression rate of P-gp in patients with ulcerative colitis (17.4%) was significantly lower than that in the control group (31.4%). The expression rate decreased to 10.1, 9.2, and 8.3% after 12, 18, and 24 months of treatment, respectively, which were significantly lower than the expression rate before treatment (17.4%). P-gp expression levels during the remission phase and active phase of ulcerative colitis were 15.2 and 17.1%, respectively, which were significantly lower than that in normal controls (31.4%). Compared with P-gp-negative patients, nitric oxide content, nitric oxide synthase activity, and interleukin-8 levels were significantly higher in P-gp-positive patients with moderately active, severely active, early onset, chronic relapsing, chronic persistent, and acute fulminant ulcerative colitis. CD4(+) and CD25(+) T cell subsets were significantly lower in the peripheral blood of patients with severely active and acute fulminant ulcerative colitis than in control subjects. Expression of the multidrug resistance gene and its product P-gp was observed in normal colon tissues and may be closely related to ulcerative colitis. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 4 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-12-19 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Colitis, Ulcerative Drug Therapy Immunology Gastrointestinal Agents Administration & Dosage Immunosuppressive Agents Intestinal Mucosa Metabolism Adolescent Case-control Studies Pathology Interleukin-8 Nitric Oxide Nitric Oxide Synthase P-glycoproteins T-lymphocyte Subsets Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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